Product Description: exosome complex component MTR3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.16.1 (Spleen exonuclease)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000177 | cytoplasmic exosome (RNase complex) |
| Component | GO:0000178 | exosome (RNase complex) |
| Component | GO:0000176 | nuclear exosome (RNase complex) |
| Component | GO:0005730 | nucleolus |
| Component | GO:0044310 | osmiophilic body |
| Process | GO:0034475 | U4 snRNA 3'-end processing |
| Process | GO:0071028 | nuclear mRNA surveillance |
| Process | GO:0034427 | nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' |
| Process | GO:0071051 | polyadenylation-dependent snoRNA 3'-end processing |
| Process | GO:0016075 | rRNA catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.10 | 0.95 |
| Trophozoite | possibly expressed | 0.20 | 0.87 |
| Schizont | possibly expressed | 0.04 | 0.97 |
| Gametocyte | not expressed | 0.16 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0209200
Resistome Missense Mutations: D265V
Resistome Compounds with Missense Mutations: Desoxyepothilone B
Resistome # Samples with Disruptive Mutations: 8 (8 missense, 8 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.262 | MFS: -2.615 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0306300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96177
"Best" AlphaFill ligand hit: 5GP (guanosine-5'-monophosphate, Local RMSD=0.60) with 2PNZ (Global RMSD=8.60)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.16.1 | spleen exonuclease | ATPKClEDTARNAiheparinarseniteATPgammaSUDP-glucosePentamidineacetylationSP-ATPalphaSSP-CTPalphaS... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102545
Most Similar Human Ortholog: Q5RKV6
TM-align score: 0.79 | RMSD: 2.41
Seq Identity: 0.21 | Length: 238 / 275
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000223496 | exosome component 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 10 | 11 | 7 |
| disruptive | 2 | 22 | 12 | 32 |
| missense | 2 | 21 | 11 | 29 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 19 | 8 | 17 |
| disruptive | 9 | 52 | 22 | 55 |
| missense | 6 | 48 | 19 | 43 |
PlasmoDB Total SNPs: 118
Non-coding: 106 | Synonymous: 9 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 275 | Molecular Weight (kDa): 31.249
UniProt ID(s): O96177
PDB ID(s): None
Isoelectric Point: 8.69
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001247 | Exoribonuclease, phosphorolytic domain 1 |
| InterPro | IPR020568 | Ribosomal protein S5 domain 2-type fold |
| PFam | PF01138 | Exoribonuclease, phosphorolytic domain 1 |
| Superfamily | SSF54211 | Ribosomal protein S5 domain 2-type fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 27432909 | Comparative Proteomics and Functional Analysis Reveal a Role of Plasmodiumfalciparum Osmiophilic Bodies in Malaria Parasite Transmission. | Suarez-Cortes P, Sharma V, ..., Alano P | 10.1074/mcp.M116.060681 |
| 30235972 | Disruption of the RNA exosome reveals the hidden face of the malaria parasitetranscriptome. | Droll D, Wei G, ..., Zhang Q | 10.1080/15476286.2018.1517014 |
| 30760080 | The anti-cancer drug 5-fluorouracil affects cell cycle regulators and potentialregulatory long non-coding RNAs in yeast | Xie B, Becker E, ..., Primig M | 10.1080/15476286.2019.1581596 |
| 23852635 | Experimental analysis of co-evolution within protein complexes: the yeast exosomeas a model | Sandler I, Medalia O, Aharoni A | 10.1002/prot.24360 |
| 36861343 | In vivo characterization of the critical interaction between the RNA exosome andthe essential RNA helicase Mtr4 in Saccharomyces cerevisiae | Sterrett MC, Farchi D, ..., Corbett AH | 10.1093/g3journal/jkad049 |
| 21919057 | Pronounced and extensive microtubule defects in a Saccharomyces cerevisiae DIS3mutant | Smith SB, Kiss DL, ..., Andrulis ED | 10.1002/yea.1899 |