PF3D7_0209700

Genome location: Pf3D7_02_v3:400,078..402,755(-)

Genome classification: Core

Function and Localization

Product Description: RING zinc finger protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Function	GO:0004842	ubiquitin-protein transferase activity
Process	GO:0007093	mitotic cell cycle checkpoint signaling
Process	GO:0016567	protein ubiquitination
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.74	0.29
Trophozoite	expressed	0.85	0.54
Schizont	expressed	0.57	0.65
Gametocyte	possibly expressed	0.91	0.51

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0209700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 3 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.128 | MFS: -2.864 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96182

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_145182

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	26	19	29
disruptive	31	62	22	101
missense	19	49	17	72

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	43	26	24
disruptive	54	125	43	141
missense	30	94	30	87

PlasmoDB Total SNPs: 110

Non-coding: 56 | Synonymous: 29 | Nonsynonymous: 25 | Stop Codon: 0

Protein Information

Protein Length: 568 | Molecular Weight (kDa): 66.706

UniProt ID(s): O96182

PDB ID(s): None

Isoelectric Point: 7.18

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001841	Zinc finger, RING-type
InterPro	IPR017907	Zinc finger, RING-type, conserved site
PFam	PF13923	N/A
Superfamily	SSF57850	N/A

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
28288121	A genetic system to study Plasmodium falciparum protein function.	Birnbaum J, Flemming S, ..., Spielmann T	10.1038/nmeth.4223