Product Description: ATP-dependent RNA helicase UAP56
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0036396 | RNA N6-methyladenosine methyltransferase complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.04 | 0.52 |
| Trophozoite | expressed | 1.20 | 0.41 |
| Schizont | expressed | 0.26 | 0.83 |
| Gametocyte | expressed | 0.60 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0209800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.257 | MFS: -2.606 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0306800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9TY94
"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.05) with 1OYY (Global RMSD=1.54)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P60842 | Eukaryotic initiation factor 4A-I | Homo sapiens | HOGENOM, OrthoDB | HippuristanolCHEMBL2058177CHEMBL2058177CHEMBL2170718... |
| P38919 | Eukaryotic initiation factor 4A-III | HOGENOM, OrthoDB | eIF4A3 inhibitor, 1eIF4A3 inhibitor, 2CHEMBL4070102CHEMBL4070102... |
Ortholog Group (OrthoMCL): OG6_101150
Most Similar Human Ortholog: A0A0D9SEM9
TM-align score: 0.93 | RMSD: 1.05
Seq Identity: 0.52 | Length: 93 / 457
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000123136 | DExD-box helicase 39A |
| ENSG00000198563 | DExD-box helicase 39B |
| ENSG00000215425 | DExD-box helicase 39B |
| ENSG00000225073 | DExD-box helicase 39B |
| ENSG00000225859 | DExD-box helicase 39B |
| ENSG00000229496 | DExD-box helicase 39B |
| ENSG00000230624 | DExD-box helicase 39B |
| ENSG00000235439 | DExD-box helicase 39B |
| ENSG00000237889 | DExD-box helicase 39B |
Protein Length: 457 | Molecular Weight (kDa): 52.224
PDB ID(s): None
Isoelectric Point: 5.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 24 | 14 | 22 |
| disruptive | 0 | 2 | 1 | 18 |
| missense | 0 | 2 | 1 | 16 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 56 | 16 | 15 |
| disruptive | 2 | 23 | 11 | 63 |
| missense | 0 | 14 | 10 | 52 |
PlasmoDB Total SNPs: 193
Non-coding: 177 | Synonymous: 16 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18722339 | Isolation and characterization of Plasmodium falciparum UAP56 homolog: evidencefor the coupling of RNA binding and splicing activity by site-directed mutations. | Shankar J, Pradhan A, Tuteja R | 10.1016/j.abb.2008.07.027 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31384004 | Transcriptome-wide dynamics of extensive m(6)A mRNA methylation during Plasmodiumfalciparum blood-stage development. | Baumgarten S, Bryant JM, ..., Scherf A | 10.1038/s41564-019-0521-7 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |