PF3D7_0210600 (CERLI1)

Genome location: Pf3D7_02_v3:431,716..434,231(-)

Genome classification: Core

Function and Localization

Product Description: protein CERLI1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0045177	apical part of cell
Component	GO:0020008	rhoptry
Component	GO:0033016	rhoptry membrane
Function	GO:0008289	lipid binding
Process	GO:0044409	entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.31	0.88
Trophozoite	expressed	0.14	0.92
Schizont	expressed	1.20	0.59
Gametocyte	expressed	1.06	0.53

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0210600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.234 | MFS: -2.616 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1002900)

Relative Growth Rate: 0.03 ± 0.06
Confidence: 6.90

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96188

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_120245

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	16	12	19
disruptive	2	24	12	40
missense	2	23	12	37

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	33	16	33
disruptive	9	51	33	87
missense	6	37	30	63

PlasmoDB Total SNPs: 165

Non-coding: 138 | Synonymous: 17 | Nonsynonymous: 8 | Stop Codon: 2

Protein Information

Protein Length: 446 | Molecular Weight (kDa): 53.675

UniProt ID(s): O96188

PDB ID(s): None

Isoelectric Point: 9.48

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
20037583	Transcriptional profiling of growth perturbations of the human malaria parasitePlasmodium falciparum.	Hu G, Cabrera A, ..., Bozdech Z	10.1038/nbt.1597
31492901	A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodiumfalciparum and Toxoplasma gondii parasites.	Suarez C, Lentini G, ..., Lebrun M	10.1038/s41467-019-11979-z
32179747	PfCERLI1 is a conserved rhoptry associated protein essential for Plasmodiumfalciparum merozoite invasion of erythrocytes.	Liffner B, Frolich S, ..., Wilson DW	10.1038/s41467-020-15127-w
35140336	Cell biological analysis reveals an essential role for Pfcerli2 in erythrocyteinvasion by malaria parasites	Liffner B, Balbin JM, ..., Wilson DW	10.1038/s42003-022-03020-9