Product Description: ras-related protein Rab-5A
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.5.2 (Small monomeric GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0030139 | endocytic vesicle |
| Component | GO:0012505 | endomembrane system |
| Component | GO:0005768 | endosome |
| Function | GO:0003924 | GTPase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006886 | intracellular protein transport |
| Process | GO:0032889 | regulation of vacuole fusion, non-autophagic |
| Process | GO:0007264 | small GTPase mediated signal transduction |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.07 | 0.97 |
| Trophozoite | expressed | 0.63 | 0.64 |
| Schizont | expressed | 0.30 | 0.81 |
| Gametocyte | expressed | 0.29 | 0.83 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0211200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.385 | MFS: -2.515 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96193
"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=0.03) with 6STF (Global RMSD=1.93)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.2 | small monomeric GTPase | GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 10 | 4 | 10 |
| disruptive | 1 | 6 | 7 | 16 |
| missense | 1 | 6 | 7 | 15 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 18 | 10 | 9 |
| disruptive | 5 | 25 | 20 | 57 |
| missense | 4 | 15 | 14 | 38 |
PlasmoDB Total SNPs: 67
Non-coding: 49 | Synonymous: 16 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 235 | Molecular Weight (kDa): 26.671
UniProt ID(s): O96193
PDB ID(s): 3CLV
Isoelectric Point: 8.81
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001806 | Small GTPase |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00071 | Small GTPase |
| PFam | PF08477 | |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12657463 | The Plasmodium falciparum family of Rab GTPases. | Quevillon E, Spielmann T, ..., Langsley G | 10.1016/s0378-1119(03)00381-0 |
| 18263733 | Four distinct pathways of hemoglobin uptake in the malaria parasite Plasmodiumfalciparum. | Elliott DA, McIntosh MT, ..., Joiner KA | 10.1073/pnas.0711067105 |
| 22188458 | Construction of a Plasmodium falciparum Rab-interactome identifies CK1 and PKA asRab-effector kinases in malaria parasites. | Rached FB, Ndjembo-Ezougou C, ..., Langsley G | 10.1111/boc.201100081 |
| 23223036 | Isoprenoid biosynthesis inhibition disrupts Rab5 localization and food vacuolarintegrity in Plasmodium falciparum. | Howe R, Kelly M, ..., Odom AR | 10.1128/EC.00073-12 |
| 29095572 | Structural plasticity mediates distinct GAP-dependent GTP hydrolysis mechanismsin Rab33 and Rab5. | Majumdar S, Acharya A, Prakash B | 10.1111/febs.14314 |
| 38022579 | Alterations of lipid-related genes during anti-tuberculosis treatment: insightsinto host immune responses and potential transcriptional biomarkers | Phat NK, Tien NTN, ..., Shin JG | 10.3389/fimmu.2023.1210372 |