Product Description: beta-ketoacyl-ACP synthase III
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.1.180 (Beta-ketoacyl-[acyl-carrier-protein] synthase III)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0033818 | beta-ketoacyl-acyl-carrier-protein synthase III activity |
| Function | GO:0005504 | fatty acid binding |
| Process | GO:0006633 | fatty acid biosynthetic process |
| Process | GO:0006631 | fatty acid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.00 | 1.00 |
| Trophozoite | possibly expressed | 0.02 | 0.99 |
| Schizont | possibly expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.54 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0211400
Resistome Missense Mutations: S150N
Resistome Compounds with Missense Mutations: BI-2536
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.855 | MFS: -2.982 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0308200)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0308200)
Modification: Disrupted | RMgm-4606
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7KWI9
"Best" AlphaFill ligand hit: COA (coenzyme a, Local RMSD=0.41) with 4X0O (Global RMSD=2.16)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.180 | beta-ketoacyl-[acyl-carrier-protein] synthase III | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P43711 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 | Haemophilus influenzae | OMA, OrthoMCL BLAST | (3-Phenoxybenzoylamino)benzoic acid deriv., 33 |
| Q2FZS0 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 | Staphylococcus aureus | OMA, OrthoMCL BLAST | (3-Phenoxybenzoylamino)benzoic acid deriv., 33 |
| P0DB00 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 | Streptococcus pyogenes | OMA, OrthoMCL BLAST | (3-Phenoxybenzoylamino)benzoic acid deriv., 24c(3-Phenoxybenzoylamino)benzoic acid deriv., 24i(3-Phenoxybenzoylamino)benzoic acid deriv., 31(3-Phenoxybenzoylamino)benzoic acid deriv., 33 |
| Q820T1 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 | Enterococcus faecalis | OMA, OrthoMCL BLAST | Diethylsulfonamide Derivative, 13aDiethylsulfonamide Derivative, 13bDiethylsulfonamide Derivative, 13eDiethylsulfonamide Derivative, 13f... |
| C3TDZ2 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 | Escherichia coli | OMA, OrthoMCL BLAST | CHEBI:17630CHEMBL3357055CHEMBL3357054CHEMBL3352863... |
| P9WNG3 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 | OMA, OrthoMCL, OrthoMCL BLAST | mtFabH inhibitor, 3mtFabH inhibitor, 4mtFabH inhibitor, 7mtFabH inhibitor, 37... | |
| O77078 | Beta-ketoacyl-[acyl-carrier-protein] synthase III | Plasmodium falciparum | OrthoMCL, OrthoMCL BLAST | CHEMBL509952CHEMBL491752CHEMBL455414CHEMBL462205... |
| A0A0C7ACN7 | 3-oxoacyl-ACP synthase | OrthoMCL BLAST | CHEMBL3397068CHEMBL3397065CHEMBL3289683CHEMBL2413670... | |
| P9WNG2 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 | Mycobacterium tuberculosis | OrthoMCL BLAST | Decyl-CoA disulfide 5 |
Protein Length: 371 | Molecular Weight (kDa): 42.325
PDB ID(s): None
Isoelectric Point: 8.64
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004655 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 |
| InterPro | IPR013747 | 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C-terminal |
| InterPro | IPR013751 | 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III |
| InterPro | IPR016039 | Thiolase-like |
| PFam | PF08541 | 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III, C-terminal |
| PFam | PF08545 | 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III |
| Superfamily | SSF53901 | Thiolase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 12 | 4 | 15 |
| disruptive | 3 | 14 | 13 | 28 |
| missense | 3 | 14 | 13 | 26 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 21 | 8 | 16 |
| disruptive | 5 | 45 | 18 | 70 |
| missense | 5 | 41 | 18 | 43 |
PlasmoDB Total SNPs: 162
Non-coding: 151 | Synonymous: 10 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 11738814 | Deciphering apicoplast targeting signals--feature extraction from nuclear-encodedprecursors of Plasmodium falciparum apicoplast proteins. | Zuegge J, Ralph S, ..., Schneider G | 10.1016/s0378-1119(01)00776-4 |
| 12270624 | Functional characterization of the acyl carrier protein (PfACP) and beta-ketoacylACP synthase III (PfKASIII) from Plasmodium falciparum. | Waters NC, Kopydlowski KM, ..., Prigge ST | 10.1016/s0166-6851(02)00140-8 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 30763089 | A KAS-III Heterodimer in Lipstatin Biosynthesis Nondecarboxylatively CondensesC(8) and C(14) Fatty Acyl-CoA Substrates by a Variable Mechanism during theEstablishment of a C(22) Aliphatic Skeleton | Zhang D, Zhang F, Liu W | 10.1021/jacs.8b12843 |
| 19407376 | Cloning, expression, crystallization and preliminary X-ray crystallographicanalysis of beta-ketoacyl-ACP synthase III (FabH) from Xanthomonas oryzae pv.oryzae | Huynh KH, Natarajan S, ..., Kang LW | 10.1107/S1744309109009555 |
| 19550039 | Cloning and sequence analysis of putative type II fatty acid synthase genes fromArachis hypogaea L | Li MJ, Li AQ, ..., Wang XJ | 10.1007/s12038-009-0027-1 |
| 18437316 | Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase III(mtFabH) assay: principles and method | Sachdeva S, Reynolds KA | 10.1007/978-1-59745-246-5_16 |
| 37995186 | Circadian clock factors regulate the first condensation reaction of fatty acidsynthesis in Arabidopsis | Kim SC, Edgeworth KN, Nusinow DA, Wang X | 10.1016/j.celrep.2023.113483 |
| 26827988 | Microbial production of bi-functional molecules by diversification of the fattyacid pathway | Garg S, Rizhsky L, ..., Nikolau BJ | 10.1016/j.ymben.2016.01.003 |
| 22753059 | Fatty acid biosynthesis in Pseudomonas aeruginosa is initiated by the FabY classof beta-ketoacyl acyl carrier protein synthases | Yuan Y, Sachdeva M, Leeds JA, Meredith TC | 10.1128/JB.00792-12 |
| 30037760 | Roles of multiple KASIII homologues of Shewanella oneidensis in initiation offatty acid synthesis and in cerulenin resistance | Meng Q, Liang H, Gao H | 10.1016/j.bbalip.2018.06.020 |
| 22753057 | Pseudomonas aeruginosa directly shunts beta-oxidation degradation intermediates intode novo fatty acid biosynthesis | Yuan Y, Leeds JA, Meredith TC | 10.1128/JB.00860-12 |
| 22017312 | Substrate recognition by beta-ketoacyl-ACP synthases | Borgaro JG, Chang A, ..., Tonge PJ | 10.1021/bi201199x |