About Gene List

PF3D7_0211500

Genome location: Pf3D7_02_v3:463,147..467,352(+)

Genome classification: Core

Function and Localization

Product Description: GAF domain-related protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 3

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.04 0.98
Trophozoite not expressed 0.22 0.87
Schizont not expressed 0.43 0.74
Gametocyte not expressed 0.46 0.74

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0211500

Resistome Missense Mutations: K617Q

Resistome Compounds with Missense Mutations: BI-2536

Resistome # Samples with Disruptive Mutations: 5 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.135 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96195

"Best" AlphaFill ligand hit: 35G (guanosine-3',5'-monophosphate, Local RMSD=0.93) with 3DBA (Global RMSD=1.60)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_131042

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 15 49 12 58
disruptive 30 185 81 203
missense 17 118 56 149

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 27 77 31 66
disruptive 97 325 149 250
missense 39 210 103 163

PlasmoDB Total SNPs: 126

Non-coding: 33 | Synonymous: 62 | Nonsynonymous: 31 | Stop Codon: 0

Protein Information

Protein Length: 1245 | Molecular Weight (kDa): 148.553

UniProt ID(s): O96195

PDB ID(s): None

Isoelectric Point: 6.68

Protein Domain Annotations:

Source Family ID Description
InterPro IPR003018 GAF domain
InterPro IPR019537 Transmembrane protein 65
PFam PF01590 GAF domain
PFam PF10507 Transmembrane protein 65
Superfamily SSF55781 N/A

Associated Publications

PMID Title Authors DOI/Link
9804551 Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Tettelin H, ..., Hoffman SL 10.1126/science.282.5391.1126
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
12368868 Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14. Gardner MJ, Shallom SJ, ..., Fraser CM 10.1038/nature01094