Product Description: tyrosine kinase-like protein, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004672 | protein kinase activity |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0007165 | signal transduction |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.78 | 0.58 |
| Schizont | possibly expressed | 0.43 | 0.74 |
| Gametocyte | possibly expressed | 0.45 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0211700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.366 | #Insertions: 7
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0308500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0308500)
Modification: Disrupted | RMgm-1780
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96197
"Best" AlphaFill ligand hit: H52 ((s)-2-methyl-1-[(4-methyl-5-isoquinoline)sulfonyl]-homopiperazine, Local RMSD=0.49) with 4F1T (Global RMSD=3.89)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_105153
Most Similar Human Ortholog: C9IYH2
TM-align score: 0.76 | RMSD: 1.85
Seq Identity: 0.05 | Length: 37 / 1233
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000073803 | mitogen-activated protein kinase kinase kinase 13 |
| ENSG00000139625 | mitogen-activated protein kinase kinase kinase 12 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 41 | 26 | 58 |
| disruptive | 82 | 170 | 80 | 171 |
| missense | 52 | 105 | 60 | 122 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 85 | 28 | 55 |
| disruptive | 148 | 285 | 110 | 240 |
| missense | 68 | 197 | 90 | 168 |
PlasmoDB Total SNPs: 304
Non-coding: 118 | Synonymous: 80 | Nonsynonymous: 106 | Stop Codon: 0
Protein Length: 1233 | Molecular Weight (kDa): 148.227
UniProt ID(s): O96197
PDB ID(s): None
Isoelectric Point: 10.07
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR001245 | Serine-threonine/tyrosine-protein kinase, catalytic domain |
| InterPro | IPR003409 | MORN motif |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR013761 | Sterile alpha motif/pointed domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF47769 | Sterile alpha motif/pointed domain superfamily |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 33608523 | 20S proteasomes secreted by the malaria parasite promote its growth. | Dekel E, Yaffe D, ..., Regev-Rudzki N | 10.1038/s41467-021-21344-8 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 32307750 | Development of new dominant selectable markers for the nonconventional yeastsOgataea polymorpha and Candida famata | Bratiichuk D, Kurylenko O, ..., Sibirny A | 10.1002/yea.3467 |
| 28352301 | Functional overexpression of genes involved in erythritol synthesis in the yeastYarrowia lipolytica | Mironczuk AM, Biegalska A, Dobrowolski A | 10.1186/s13068-017-0772-6 |
| 29729128 | A High-throughput workflow for CRISPR/Cas9 mediated combinatorial promoterreplacements and phenotype characterization in yeast | Kuivanen J, Holmstrom S, ..., Jantti J | 10.1002/biot.201700593 |
| 34592433 | Crabtree/Warburg-like aerobic xylose fermentation by engineered Saccharomycescerevisiae | Lee SB, Tremaine M, ..., Sato TK | 10.1016/j.ymben.2021.09.008 |
| 30252062 | Reassessment of requirements for anaerobic xylose fermentation by engineered,non-evolved Saccharomyces cerevisiae strains | Bracher JM, Martinez-Rodriguez OA, ..., Pronk JT | 10.1093/femsyr/foy104 |
| 30711353 | Peroxisomal Fba2p and Tal2p complementally function in the rearrangement pathwayfor xylulose 5-phosphate in the methylotrophic yeast Pichia pastoris | Fukuoka H, Kawase T, ..., Nakagawa T | 10.1016/j.jbiosc.2019.01.008 |
| 30586497 | Metabolic Engineering of Saccharomyces cerevisiae for Production of Shinorine, aSunscreen Material, from Xylose | Park SH, Lee K, Jang JW, Hahn JS | 10.1021/acssynbio.8b00388 |
| 31996457 | Toxoplasma gondii tkl1 Deletion Mutant Is a Promising Vaccine against Acute,Chronic, and Congenital Toxoplasmosis in Mice | Wang JL, Liang QL, ..., Zhu XQ | 10.4049/jimmunol.1900410 |
| 28181081 | Genetic improvement of xylose metabolism by enhancing the expression of pentosephosphate pathway genes in Saccharomyces cerevisiae IR-2 for high-temperatureethanol production | Kobayashi Y, Sahara T, ..., Fujimori KE | 10.1007/s10295-017-1912-5 |
| 30034524 | Peroxisomes and peroxisomal transketolase and transaldolase enzymes are essentialfor xylose alcoholic fermentation by the methylotrophic thermotolerant yeast,Ogataea (Hansenula) polymorpha | Kurylenko OO, Ruchala J, ..., Sibirny AA | 10.1186/s13068-018-1203-z |
| 29621349 | Signature pathway expression of xylose utilization in the genetically engineeredindustrial yeast Saccharomyces cerevisiae | Feng Q, Liu ZL, Weber SA, Li S | 10.1371/journal.pone.0195633 |
| 31863218 | Comparative transcriptome analysis of genomic region deletion strain withenhanced L-tyrosine production in Saccharomyces cerevisiae | Wu Y, Cai M, ..., Qiao M | 10.1007/s10529-019-02784-1 |
| 30151682 | Systematic optimization of gene expression of pentose phosphate pathway enhancesethanol production from a glucose/xylose mixed medium in a recombinantSaccharomyces cerevisiae | Kobayashi Y, Sahara T, ..., Fujimori KE | 10.1186/s13568-018-0670-8 |
| 32653007 | The influence of transketolase on lipid biosynthesis in the yeast Yarrowialipolytica | Dobrowolski A, Mironczuk AM | 10.1186/s12934-020-01398-x |