Product Description: asparagine--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.22 (Asparagine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0004816 | asparagine-tRNA ligase activity |
| Process | GO:0006421 | asparaginyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.48 | 0.78 |
| Trophozoite | expressed | 1.09 | 0.45 |
| Schizont | expressed | 0.29 | 0.81 |
| Gametocyte | expressed | 0.53 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0211800
Resistome Missense Mutations: R487S
Resistome Compounds with Missense Mutations: OSM-S-106
Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.184 | MFS: -2.765 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1109200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0308600)
Modification: Disrupted | RMgm-1781
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96198
"Best" AlphaFill ligand hit: DSZ (5'-o-(l-alpha-aspartylsulfamoyl)adenosine, Local RMSD=0.55) with 6SJC (Global RMSD=5.69)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.22 | asparagine-tRNA ligase | 3D6NH4+NO3-CNS-LCM01LCM02CH3COO-NSC12156NSC35467NSC114691NSC363624variolin B... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101288
Most Similar Human Ortholog: Q96I59
TM-align score: 0.90 | RMSD: 1.91
Seq Identity: 0.41 | Length: 449 / 610
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000137513 | asparaginyl-tRNA synthetase 2, mitochondrial |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 38 | 13 | 42 |
| disruptive | 8 | 29 | 15 | 61 |
| missense | 7 | 25 | 15 | 54 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 78 | 17 | 43 |
| disruptive | 12 | 71 | 40 | 105 |
| missense | 7 | 59 | 37 | 74 |
PlasmoDB Total SNPs: 73
Non-coding: 54 | Synonymous: 15 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 610 | Molecular Weight (kDa): 70.498
UniProt ID(s): O96198
PDB ID(s): None
Isoelectric Point: 6.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002312 | Aspartyl/Asparaginyl-tRNA synthetase, class IIb |
| InterPro | IPR004364 | Aminoacyl-tRNA synthetase, class II (D/K/N) |
| InterPro | IPR004365 | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type |
| InterPro | IPR004522 | Asparagine-tRNA ligase |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| PFam | PF00152 | Aminoacyl-tRNA synthetase, class II (D/K/N) |
| PFam | PF01336 | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF55681 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24196969 | Aminoacylation of Plasmodium falciparum tRNA(Asn) and insights in the synthesisof asparagine repeats. | Filisetti D, Theobald-Dietrich A, ..., Frugier M | 10.1074/jbc.M113.522896 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 37546892 | Reaction hijacking inhibition of Plasmodium falciparum asparagine tRNAsynthetase | Xie SC, Wang Y, ..., Tilley L | 10.21203/rs.3.rs-3198291/v1 |
| 37572327 | Solution structure of the N-terminal extension domain of a Schistosoma japonicumasparaginyl-tRNA synthetase | Peck Y, Pickering D, ..., Daly NL | 10.1080/07391102.2023.2241918 |
| 26804570 | The Bacillus subtilis and Bacillus halodurans Aspartyl-tRNA Synthetases RetainRecognition of tRNA(Asn) | Nair N, Raff H, ..., Sheppard K | 10.1016/j.jmb.2016.01.014 |
| 28180287 | The complex evolutionary history of aminoacyl-tRNA synthetases | Chaliotis A, Vlastaridis P, ..., Amoutzias GD | 10.1093/nar/gkw1182 |
| 27223819 | Aminoacyl-tRNA Synthetases in the Bacterial World | Giege R, Springer M | 10.1128/ecosalplus.ESP-0002-2016 |
| 33528599 | Structural and Genetic Determinants of Convergence in the Drosophila tRNAStructure-Function Map | Phillips JB, Ardell DH | 10.1007/s00239-021-09995-z |
| 35634293 | Aminoacyl-tRNA Synthetases: On Anti-Synthetase Syndrome and Beyond | Galindo-Feria AS, Notarnicola A, Lundberg IE, Horuluoglu B | 10.3389/fimmu.2022.866087 |
| 38297033 | Reaction hijacking inhibition of Plasmodium falciparum asparagine tRNAsynthetase | Xie SC, Wang Y, ..., Tilley L | 10.1038/s41467-024-45224-z |
| 30919766 | Spectroscopic Studies of Asparaginyl-tRNA Synthetase from Entamoeba histolytica | Biswas P, Sahu DK, Sahu K, Banerjee R | 10.2174/0929866526666190327122419 |