Product Description: eukaryotic peptide chain release factor subunit 1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.1.29 (Aminoacyl-tRNA hydrolase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Component | GO:0018444 | translation release factor complex |
| Function | GO:1990825 | sequence-specific mRNA binding |
| Function | GO:0003747 | translation release factor activity |
| Function | GO:0016149 | translation release factor activity, codon specific |
| Process | GO:0002184 | cytoplasmic translational termination |
| Process | GO:0006449 | regulation of translational termination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.79 | 0.63 |
| Trophozoite | expressed | 0.83 | 0.56 |
| Schizont | expressed | 0.09 | 0.95 |
| Gametocyte | expressed | 0.63 | 0.65 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0212300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.662 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96203
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.98) with 3E1Y (Global RMSD=4.23)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.1.29 | aminoacyl-tRNA hydrolase | EDTAtRNAtRNALystRNAfMettRNA-lysine5-azacytidine70 S ribosomeUncharged tRNAdeacetylated tRNAdiethyldicarbonatenon-esterified tRNALystRNA-formyl-methionine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100994
Most Similar Human Ortholog: P62495
TM-align score: 0.97 | RMSD: 1.48
Seq Identity: 0.68 | Length: 425 / 427
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000120705 | eukaryotic translation termination factor 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 23 | 11 | 15 |
| disruptive | 0 | 1 | 2 | 8 |
| missense | 0 | 1 | 2 | 4 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 43 | 13 | 12 |
| disruptive | 2 | 11 | 7 | 30 |
| missense | 1 | 4 | 6 | 19 |
PlasmoDB Total SNPs: 143
Non-coding: 137 | Synonymous: 5 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 427 | Molecular Weight (kDa): 48.064
UniProt ID(s): O96203
PDB ID(s): None
Isoelectric Point: 6.97
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004403 | Peptide chain release factor eRF1/aRF1 |
| InterPro | IPR005140 | eRF1 domain 1/Pelota-like |
| InterPro | IPR005141 | eRF1 domain 2 |
| InterPro | IPR005142 | eRF1 domain 3 |
| InterPro | IPR024049 | Peptide Chain Release Factor eRF1/aRF1, N-terminal |
| InterPro | IPR029064 | 50S ribosomal protein L30e-like |
| PFam | PF03463 | eRF1 domain 1/Pelota-like |
| PFam | PF03464 | eRF1 domain 2 |
| PFam | PF03465 | eRF1 domain 3 |
| Superfamily | SSF53137 | 50S ribosomal protein L30e-like |
| Superfamily | SSF55315 | Peptide Chain Release Factor eRF1/aRF1, N-terminal |
| Superfamily | SSF55481 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |