Product Description: secreted protein with altered thrombospondin repeat domain
SignalP Peptide: MKKSRFLLLSIFFCFVTNISL
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0045177 | apical part of cell |
| Component | GO:0009986 | cell surface |
| Component | GO:0020009 | microneme |
| Component | GO:0020008 | rhoptry |
| Process | GO:0098609 | cell-cell adhesion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.42 | 0.85 |
| Trophozoite | expressed | 0.16 | 0.90 |
| Schizont | expressed | 0.58 | 0.82 |
| Gametocyte | possibly expressed | 0.08 | 0.95 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0212600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.966 | MFS: -2.553 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0309500)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0309500)
Modification: Tagged | RMgm-5185
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96207
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 15 | 3 | 10 |
| disruptive | 1 | 16 | 9 | 22 |
| missense | 1 | 16 | 9 | 21 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 20 | 9 | 25 |
| disruptive | 6 | 33 | 23 | 42 |
| missense | 5 | 28 | 20 | 31 |
PlasmoDB Total SNPs: 122
Non-coding: 118 | Synonymous: 4 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 250 | Molecular Weight (kDa): 29.218
UniProt ID(s): O96207
PDB ID(s): None
Isoelectric Point: 7.83
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR036383 | Thrombospondin type-1 (TSP1) repeat superfamily |
| PFam | N/A | N/A |
| Superfamily | SSF82895 | Thrombospondin type-1 (TSP1) repeat superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12716913 | PfSPATR, a Plasmodium falciparum protein containing an altered thrombospondintype I repeat domain is expressed at several stages of the parasite life cycleand is the target of inhibitory antibodies. | Chattopadhyay R, Rathore D, ..., Hoffman SL | 10.1074/jbc.M300865200 |
| 18832006 | Identification of peptides with high red blood cell and hepatocyte bindingactivity in the Plasmodium falciparum multi-stage invasion proteins: PfSPATR andMCP-1. | Curtidor H, Garcia J, ..., Patarroyo ME | 10.1016/j.biochi.2008.08.003 |
| 20037583 | Transcriptional profiling of growth perturbations of the human malaria parasitePlasmodium falciparum. | Hu G, Cabrera A, ..., Bozdech Z | 10.1038/nbt.1597 |
| 25092910 | A conserved apicomplexan microneme protein contributes to Toxoplasma gondiiinvasion and virulence. | Huynh MH, Boulanger MJ, Carruthers VB | 10.1128/IAI.01877-14 |
| 34252120 | Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccineantigens using chimeric rodent parasites | Kolli SK, Salman AM, ..., Janse CJ | 10.1371/journal.pone.0254498 |
| 37886905 | A conserved complex of microneme proteins mediates rhoptry discharge inToxoplasma | Valleau D, Sidik SM, ..., Lourido S | 10.15252/embj.2022113155 |
| 17041140 | Developmental biology of sporozoite-host interactions in Plasmodium falciparummalaria: implications for vaccine design | Garcia JE, Puentes A, Patarroyo ME | 10.1128/CMR.00063-05 |
| 36602208 | Genetic diversity of secreted protein with an altered thrombospondin repeat(SPATR) of Plasmodium knowlesi clinical isolates from Malaysia | Azlan UW, Lau YL, ..., Fong MY | 10.47665/tb.39.4.006 |
| 17880006 | Proteomic analysis of calcium-dependent secretion in Toxoplasma gondii | Kawase O, Nishikawa Y, ..., Xuan X | 10.1002/pmic.200700362 |
| 35594144 | C-Mannosyltransferase Is Essential for Malaria Transmission in Plasmodiumberghei | Srivastava PN, Nayak B, Dewaker V, Mishra S | 10.1021/acsinfecdis.2c00239 |
| 16113256 | Identification, cloning, expression, and characterization of the gene forPlasmodium knowlesi surface protein containing an altered thrombospondin repeatdomain | Mahajan B, Jani D, ..., Rathore D | 10.1128/IAI.73.9.5402-5409.2005 |
| 20144733 | Characterization of a novel thrombospondin-related protein in Toxoplasma gondii | Kawase O, Nishikawa Y, ..., Xuan X | 10.1016/j.parint.2010.02.001 |
| 31755154 | Secreted protein with altered thrombospondin repeat (SPATR) is essential forasexual blood stages but not required for hepatocyte invasion by the malariaparasite Plasmodium berghei | Gupta R, Mishra A, ..., Mishra S | 10.1111/mmi.14432 |
| 23734702 | Cloning, expression, and immunocharacterization of surface protein containing analtered thrombospondin repeat domain (SPATR) from Plasmodium knowlesi | Palaeya V, Lau YL, ..., Fong MY | 10.1186/1475-2875-12-182 |