PF3D7_0212800 (MATE)

Genome location: Pf3D7_02_v3:526,387..531,923(+)

Genome classification: Core

Function and Localization

Product Description: multidrug efflux pump, putative

SignalP Peptide: N/A

# Transmembrane Domains: 11

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016020	membrane
Function	GO:0022857	transmembrane transporter activity
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	not expressed	0.28	0.83
Schizont	not expressed	0.08	0.94
Gametocyte	not expressed	0.85	0.55

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0212800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 3 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.497 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0309700)

Relative Growth Rate: 0.92 ± 0.24
Confidence: 4.22

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0309700)

Modification: Disrupted | RMgm-1786

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96209

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533426

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	48	25	62
disruptive	44	146	58	188
missense	28	123	51	164

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	95	50	72
disruptive	81	254	112	232
missense	50	215	86	179

PlasmoDB Total SNPs: 305

Non-coding: 162 | Synonymous: 91 | Nonsynonymous: 52 | Stop Codon: 0

Protein Information

Protein Length: 1224 | Molecular Weight (kDa): 144.032

UniProt ID(s): O96209

PDB ID(s): None

Isoelectric Point: 8.38

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002528	Multi antimicrobial extrusion protein
PFam	PF01554	Multi antimicrobial extrusion protein
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
29326268	Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics.	Cowell AN, Istvan ES, ..., Winzeler EA	10.1126/science.aan4472