PF3D7_0212900 (ATEL1)

Genome location: Pf3D7_02_v3:532,001..534,657(+)

Genome classification: Core

Function and Localization

Product Description: arginyl-tRNA--protein transferase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.3.2.8 (Arginyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0004057	arginyltransferase activity
Function	GO:0003824	catalytic activity
Function	GO:0008914	leucyltransferase activity
Process	GO:0016598	protein arginylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.05	0.98
Trophozoite	expressed	0.46	0.72
Schizont	expressed	0.64	0.61
Gametocyte	expressed	0.25	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0212900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -0.947 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0309800)

Relative Growth Rate: 0.79 ± 0.14
Confidence: 5.30

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0309800)

Modification: Disrupted | RMgm-1787

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96210

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.2.8	arginyltransferase	heminDNAseRNaseheparinsuraminsperminemerbrominspermidineputrescineDisulfiramTannic acidisoglutamine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_108194

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	13	3	19
disruptive	2	9	6	22
missense	2	9	5	16

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	25	4	29
disruptive	8	26	15	43
missense	4	18	11	32

PlasmoDB Total SNPs: 135

Non-coding: 130 | Synonymous: 5 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 365 | Molecular Weight (kDa): 42.94

UniProt ID(s): O96210, Q195H6

PDB ID(s): None

Isoelectric Point: 7.6

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004616	Leucyl/phenylalanyl-tRNA-protein transferase
InterPro	IPR016181	Acyl-CoA N-acyltransferase
PFam	PF03588	Leucyl/phenylalanyl-tRNA-protein transferase
Superfamily	SSF55729	Acyl-CoA N-acyltransferase

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16492767	Aminoacyl-transferases and the N-end rule pathway of prokaryotic/eukaryoticspecificity in a human pathogen.	Graciet E, Hu RG, ..., Varshavsky A	10.1073/pnas.0511224103