Product Description: protein SIS1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0051087 | chaperone binding |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0051085 | chaperone cofactor-dependent protein refolding |
| Process | GO:0006457 | protein folding |
| Process | GO:0009408 | response to heat |
| Process | GO:0006986 | response to unfolded protein |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.32 | 0.84 |
| Trophozoite | expressed | 0.79 | 0.57 |
| Schizont | expressed | 0.10 | 0.93 |
| Gametocyte | expressed | 0.82 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0213100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.238 | MFS: -2.617 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0310000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0310000)
Modification: Disrupted | RMgm-1788
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96212
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P31689 | DnaJ homolog subfamily A member 1 | Homo sapiens | OrthoDB | CHEMBL52073CHEMBL159171 |
Ortholog Group (OrthoMCL): OG6_100302
Most Similar Human Ortholog: H0Y4S1
TM-align score: 0.86 | RMSD: 0.70
Seq Identity: 0.55 | Length: 33 / 328
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000132002 | DnaJ heat shock protein family (Hsp40) member B1 |
| ENSG00000137094 | DnaJ heat shock protein family (Hsp40) member B5 |
| ENSG00000162616 | DnaJ heat shock protein family (Hsp40) member B4 |
| ENSG00000187726 | DnaJ heat shock protein family (Hsp40) member B13 |
Protein Length: 328 | Molecular Weight (kDa): 37.356
UniProt ID(s): O96212
PDB ID(s): None
Isoelectric Point: 9.17
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001623 | DnaJ domain |
| InterPro | IPR002939 | Chaperone DnaJ, C-terminal |
| InterPro | IPR008971 | HSP40/DnaJ peptide-binding |
| InterPro | IPR018253 | DnaJ domain, conserved site |
| InterPro | IPR036869 | Chaperone J-domain superfamily |
| PFam | PF00226 | DnaJ domain |
| PFam | PF01556 | Chaperone DnaJ, C-terminal |
| Superfamily | SSF46565 | Chaperone J-domain superfamily |
| Superfamily | SSF49493 | HSP40/DnaJ peptide-binding |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 15 | 11 | 24 |
| disruptive | 0 | 3 | 5 | 18 |
| missense | 0 | 3 | 4 | 16 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 36 | 19 | 29 |
| disruptive | 2 | 18 | 15 | 75 |
| missense | 0 | 12 | 9 | 57 |
PlasmoDB Total SNPs: 112
Non-coding: 103 | Synonymous: 8 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 25701168 | PFB0595w is a Plasmodium falciparum J protein that co-localizes with PfHsp70-1and can stimulate its in vitro ATP hydrolysis activity. | Njunge JM, Mandal P, ..., Blatch GL | 10.1016/j.biocel.2015.02.008 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 37589820 | Backbone and sidechain NMR assignments of residues 1-81 from yeast Sis1 incomplex with an Hsp70 C-terminal EEVD peptide | Matos CO, Pinheiro GMS, Ramos CHI, Almeida FCL | 10.1007/s12104-023-10148-0 |
| 34849884 | Yeast J-protein Sis1 prevents prion toxicity by moderating depletion of prionprotein | Kumar J, Reidy M, Masison DC | 10.1093/genetics/iyab129 |
| 34935410 | Essentiality of Sis1, a J-domain protein Hsp70 cochaperone, can be overcome byTti1, a specialized PIKK chaperone | Schilke BA, Craig EA | 10.1091/mbc.E21-10-0493 |
| 36576197 | Identification of subfunctionalized aggregate-remodeling J-domain proteins inArabidopsis thaliana | Tak Y, Lal SS, ..., Sahi C | 10.1093/jxb/erac514 |
| 36298715 | Hsp40/JDP Requirements for the Propagation of Synthetic Yeast Prions | Miller SC, Wegrzynowicz AK, ..., Hines JK | 10.3390/v14102160 |
| 37158601 | Transcriptional regulation of Sis1 promotes fitness but not feedback in the heatshock response | Garde R, Singh A, Ali A, Pincus D | 10.7554/eLife.79444 |
| 36138748 | Anti-Prion Systems Block Prion Transmission, Attenuate Prion Generation, CureMost Prions as They Arise and Limit Prion-Induced Pathology in Saccharomycescerevisiae | Wickner RB, Edskes HK, Son M, Wu S | 10.3390/biology11091266 |
| 35205876 | Differential Interactions of Molecular Chaperones and Yeast Prions | Barbitoff YA, Matveenko AG, Zhouravleva GA | 10.3390/jof8020122 |
| 37903258 | Human proteins curing yeast prions | Wu S, Edskes HK, Wickner RB | 10.1073/pnas.2314781120 |
| 38331212 | Role of J-domain Proteins in Yeast Physiology and Protein Quality Control | Ruger-Herreros C, Svoboda L, Mogk A, Bukau B | 10.1016/j.jmb.2024.168484 |
| 35093637 | Elevated constitutive expression of Hsp40 chaperone Sis1 reduces TDP-43aggregation-induced oxidative stress in Ire1 pathway dependent-manner in yeastTDP-43 proteinopathy model of amyotrophic lateral sclerosis | Bharathi V, Bajpai A, Parappuram IT, Patel BK | 10.1016/j.bbrc.2022.01.073 |
| 34873058 | Class-specific interactions between Sis1 J-domain protein and Hsp70 chaperonepotentiate disaggregation of misfolded proteins | Wyszkowski H, Janta A, ..., Liberek K | 10.1073/pnas.2108163118 |
| 35177839 | Amyloid conformation-dependent disaggregation in a reconstituted yeast prionsystem | Nakagawa Y, Shen HC, ..., Tanaka M | 10.1038/s41589-021-00951-y |
| 36552355 | Human J-Domain Protein DnaJB6 Protects Yeast from [PSI(+)] Prion Toxicity | Dolder RE 3rd, Kumar J, Reidy M, Masison DC | 10.3390/biology11121846 |
| 35931773 | Disease-associated mutations within the yeast DNAJB6 homolog Sis1 slowconformer-specific substrate processing and can be corrected by the modulation ofnucleotide exchange factors | Bhadra AK, Rau MJ, ..., True HL | 10.1038/s41467-022-32318-9 |