Product Description: protein kinase 7
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0004708 | MAP kinase kinase activity |
| Function | GO:0004672 | protein kinase activity |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0020014 | schizogony |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.98 |
| Trophozoite | expressed | 0.25 | 0.84 |
| Schizont | expressed | 0.04 | 0.97 |
| Gametocyte | expressed | 0.14 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0213400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.959 | #Insertions: 2
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0310300)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0310300)
Modification: Disrupted | RMgm-524
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7YTF7
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.41) with 2PML (Global RMSD=1.38)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O96214 | Protein kinase domain-containing protein | Plasmodium falciparum | OrthoMCL, OrthoMCL BLAST | CHEMBL524106CHEMBL450347CHEMBL522222CHEMBL496113... |
Protein Length: 343 | Molecular Weight (kDa): 40.963
Isoelectric Point: 8.55
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 20 | 15 | 21 |
| disruptive | 6 | 18 | 12 | 34 |
| missense | 6 | 18 | 11 | 30 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 37 | 12 | 39 |
| disruptive | 7 | 49 | 35 | 69 |
| missense | 6 | 41 | 32 | 49 |
PlasmoDB Total SNPs: 0
Non-coding: 0 | Synonymous: 0 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 15612927 | PfPK7, an atypical MEK-related protein kinase, reflects the absence of classicalthree-component MAPK pathways in the human malaria parasite Plasmodiumfalciparum. | Dorin D, Semblat JP, ..., Doerig C | 10.1111/j.1365-2958.2004.04393.x |
| 18083830 | Disruption of the PfPK7 gene impairs schizogony and sporogony in the humanmalaria parasite Plasmodium falciparum. | Dorin-Semblat D, Sicard A, ..., Doerig C | 10.1128/EC.00245-07 |
| 29480948 | Melatonin activates FIS1, DYN1, and DYN2 Plasmodium falciparum related-genes formitochondria fission: Mitoemerald-GFP as a tool to visualize mitochondriastructure. | Scarpelli PH, Tessarin-Almeida G, ..., Garcia CRS | 10.1111/jpi.12484 |
| 29678115 | Characterization of Plasmodium falciparum Atypical Kinase PfPK7(-) DependentPhosphoproteome. | Pease BN, Huttlin EL, ..., Chakrabarti D | 10.1021/acs.jproteome.8b00062 |
| 33247522 | Artificial Intelligence Applied to the Rapid Identification of New Antimalarial Candidates with Dual-Stage Activity | Lima MNN, Borba JVB, ..., Andrade CH | 10.1002/cmdc.202000685 |
| 26934697 | Biochemical Screening of Five Protein Kinases from Plasmodium falciparum against 14,000 Cell-Active Compounds | Crowther GJ, Hillesland HK, ..., Ojo KK | 10.1371/journal.pone.0149996 |