About Gene List

PF3D7_0215000 (ACS9)

Genome location: Pf3D7_02_v3:615,813..621,692(-)

Genome classification: Core

Function and Localization

Product Description: acyl-CoA synthetase

SignalP Peptide: MLIYNFFIVLIYIFQTAS

# Transmembrane Domains: 0

EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005783 endoplasmic reticulum
Component GO:0016020 membrane
Function GO:0004467 long-chain fatty acid-CoA ligase activity
Process GO:0006631 fatty acid metabolic process
Process GO:0015909 long-chain fatty acid transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.11 0.95
Trophozoite expressed 0.56 0.69
Schizont expressed 0.24 0.85
Gametocyte expressed 1.51 0.33

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0215000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.232 | #Insertions: 3

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)

  • Relative Growth Rate: 0.41 ± 0.29
  • Confidence: 3.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96230

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.07) with 3E7X (Global RMSD=4.71)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
6.2.1.3 long-chain-fatty-acid-CoA ligase ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100152

Most Similar Human Ortholog: B7Z3Z9

TM-align score: 0.89 | RMSD: 2.94

Seq Identity: 0.23 | Length: 516 / 885

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000151726 acyl-CoA synthetase long chain family member 1
ENSG00000164398 acyl-CoA synthetase long chain family member 6
ENSG00000197142 acyl-CoA synthetase long chain family member 5
ENSG00000281938 novel transcript

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 4 34 15 43
disruptive 48 86 26 68
missense 29 60 19 59

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 10 60 24 59
disruptive 80 152 79 202
missense 46 101 59 117

PlasmoDB Total SNPs: 367

Non-coding: 276 | Synonymous: 51 | Nonsynonymous: 38 | Stop Codon: 2

Protein Information

Protein Length: 885 | Molecular Weight (kDa): 102.147

UniProt ID(s): O96230

PDB ID(s): None

Isoelectric Point: 4.91

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000873 AMP-dependent synthetase/ligase
InterPro IPR020845 AMP-binding, conserved site
PFam PF00501 AMP-dependent synthetase/ligase
Superfamily SSF56801 N/A

Associated Publications

PMID Title Authors DOI/Link
9804551 Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Tettelin H, ..., Hoffman SL 10.1126/science.282.5391.1126
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097