About Gene List

PF3D7_0215800 (ORC5)

Genome location: Pf3D7_02_v3:650,472..653,693(-)

Genome classification: Core

Function and Localization

Product Description: origin recognition complex subunit 5

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005664 nuclear origin of replication recognition complex
Component GO:0005656 nuclear pre-replicative complex
Function GO:0005524 ATP binding
Function GO:0016887 ATP hydrolysis activity
Function GO:0003688 DNA replication origin binding
Process GO:0006270 DNA replication initiation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.04 0.98
Trophozoite expressed 0.31 0.80
Schizont expressed 0.10 0.93
Gametocyte expressed 0.32 0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0215800

Resistome Missense Mutations: K72T

Resistome Compounds with Missense Mutations: Atovaquone

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.128 | MFS: -2.848 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0312500)

  • Relative Growth Rate: 0.20 ± 0.24
  • Confidence: 4.24

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96237

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=1.80) with 6S2P (Global RMSD=7.90)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_185965

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 14 40 30 60
disruptive 34 69 36 121
missense 17 48 25 104

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 20 96 39 62
disruptive 61 136 102 203
missense 32 94 87 139

PlasmoDB Total SNPs: 106

Non-coding: 21 | Synonymous: 54 | Nonsynonymous: 30 | Stop Codon: 1

Protein Information

Protein Length: 899 | Molecular Weight (kDa): 103.992

UniProt ID(s): O96237

PDB ID(s): None

Isoelectric Point: 4.65

Protein Domain Annotations:

Source Family ID Description
InterPro IPR020796 Origin recognition complex, subunit 5
InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
PFam PF14630 Origin recognition complex, subunit 5
Superfamily SSF52540 P-loop containing nucleoside triphosphate hydrolase

Associated Publications

PMID Title Authors DOI/Link
9804551 Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Tettelin H, ..., Hoffman SL 10.1126/science.282.5391.1126
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
18554328 Plasmodium falciparum origin recognition complex subunit 5: functionalcharacterization and role in DNA replication foci formation. Gupta A, Mehra P, Dhar SK 10.1111/j.1365-2958.2008.06316.x
34174709 Set1-dependent H3K4 methylation becomes critical for limiting DNA damage inresponse to changes in S-phase dynamics in Saccharomyces cerevisiae de La Roche Saint-Andre C, Geli V 10.1016/j.dnarep.2021.103159