PF3D7_0215900 (DHHC11)

Genome location: Pf3D7_02_v3:654,918..656,840(-)

Genome classification: Core

Function and Localization

Product Description: palmitoyltransferase DHHC11, putative

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0005783	endoplasmic reticulum
Function	GO:0016409	palmitoyltransferase activity
Function	GO:0019706	protein-cysteine S-palmitoyltransferase activity
Function	GO:0008270	zinc ion binding
Process	GO:0018230	peptidyl-L-cysteine S-palmitoylation
Process	GO:0018345	protein palmitoylation
Process	GO:0006612	protein targeting to membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.00	1.00
Trophozoite	expressed	0.06	0.96
Schizont	expressed	0.10	0.92
Gametocyte	expressed	0.12	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0215900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.995 | MFS: -2.633 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0312600)

Relative Growth Rate: 0.96 ± 0.21
Confidence: 4.50

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0312600)

Modification: Disrupted | RMgm-1804

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96238

"Best" AlphaFill ligand hit: PEE (dope, Local RMSD=0.07) with 1PP9 (Global RMSD=16.07)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.-	Ex: [myelin-proteolipid] O-palmitoyltransferase	CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.225	protein S-acyltransferase	stearoyl-CoAmyristoyl-CoA2-Bromopalmitate2-bromo-palmitateacyl-CoA binding protein

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9UIJ5	Palmitoyltransferase ZDHHC2		OrthoDB	CHEMBL118417CHEMBL5201039CHEMBL5199102CHEMBL5186633
Q5W0Z9	Palmitoyltransferase ZDHHC20		OrthoDB	CHEMBL118417CHEMBL5201039CHEMBL5186346CHEMBL5205503...

Orthology Information

Ortholog Group (OrthoMCL): OG6_104274

Most Similar Human Ortholog: C9JNU5

TM-align score: 0.92 | RMSD: 0.62

Seq Identity: 0.41 | Length: 44 / 260

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000153786	zinc finger DHHC-type palmitoyltransferase 7
ENSG00000163812	zinc finger DHHC-type palmitoyltransferase 3

Protein Information

Protein Length: 260 | Molecular Weight (kDa): 30.829

UniProt ID(s): O96238

PDB ID(s): None

Isoelectric Point: 9.04

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001594	Palmitoyltransferase, DHHC domain
PFam	PF01529	Palmitoyltransferase, DHHC domain
Superfamily	N/A	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	11	10	17
disruptive	5	19	22	31
missense	5	19	20	29

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	27	12	19
disruptive	9	51	39	59
missense	9	42	32	41

PlasmoDB Total SNPs: 108

Non-coding: 88 | Synonymous: 14 | Nonsynonymous: 6 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
12368868	Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14.	Gardner MJ, Shallom SJ, ..., Fraser CM	10.1038/nature01094