Product Description: vacuolar protein sorting-associated protein 45
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000139 | Golgi membrane |
| Component | GO:0005737 | cytoplasm |
| Process | GO:0098657 | import into cell |
| Process | GO:0006886 | intracellular protein transport |
| Process | GO:0015031 | protein transport |
| Process | GO:0016192 | vesicle-mediated transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.38 | 0.77 |
| Schizont | expressed | 0.25 | 0.84 |
| Gametocyte | expressed | 0.51 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0216400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -2.861 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0313100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96243
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101522
Most Similar Human Ortholog: B7Z7G7
TM-align score: 0.93 | RMSD: 1.62
Seq Identity: 0.31 | Length: 198 / 722
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000136631 | vacuolar protein sorting 45 homolog |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 43 | 26 | 39 |
| disruptive | 11 | 52 | 32 | 100 |
| missense | 8 | 39 | 27 | 65 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 74 | 33 | 48 |
| disruptive | 32 | 126 | 91 | 222 |
| missense | 17 | 89 | 64 | 130 |
PlasmoDB Total SNPs: 104
Non-coding: 65 | Synonymous: 26 | Nonsynonymous: 13 | Stop Codon: 0
Protein Length: 722 | Molecular Weight (kDa): 86.256
UniProt ID(s): O96243
PDB ID(s): None
Isoelectric Point: 6.97
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001619 | Sec1-like protein |
| InterPro | IPR036045 | Sec1-like superfamily |
| PFam | PF00995 | Sec1-like protein |
| Superfamily | SSF56815 | Sec1-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30581113 | PfVPS45 Is Required for Host Cell Cytosol Uptake by Malaria Blood StageParasites. | Jonscher E, Flemming S, ..., Spielmann T | 10.1016/j.chom.2018.11.010 |
| 33512427 | Mammalian VPS45 orchestrates trafficking through the endosomal system | Frey L, Zietara N, ..., Klein C | 10.1182/blood.2020006871 |
| 36923123 | VPS45 is required for both diffuse and tip growth of Arabidopsis thaliana cells | Mugume Y, Roy R, ..., Bassham DC | 10.3389/fpls.2023.1120307 |
| 38089934 | A humanized Caenorhabditis elegans model for studying pathogenic mutations inVPS45, a protein essential for membrane trafficking, associated with severecongenital neutropenia | Gengyo-Ando K, Tateyama M, ..., Nakai J | 10.17912/micropub.biology.001052 |