Product Description: ATP synthase subunit alpha, mitochondrial
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.3.14 (Transferred entry: 7.1.2.2);7.1.2.2 (H(+)-transporting two-sector ATPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005886 | plasma membrane |
| Component | GO:0045261 | proton-transporting ATP synthase complex, catalytic core F(1) |
| Function | GO:0043531 | ADP binding |
| Function | GO:0005524 | ATP binding |
| Function | GO:0046933 | proton-transporting ATP synthase activity, rotational mechanism |
| Process | GO:0015986 | ATP synthesis coupled proton transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.12 | 0.94 |
| Trophozoite | expressed | 0.77 | 0.58 |
| Schizont | expressed | 0.41 | 0.75 |
| Gametocyte | expressed | 0.80 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0217100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.141 | MFS: -2.779 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0313800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0313800)
Modification: Disrupted | RMgm-1811
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96252
"Best" AlphaFill ligand hit: B3P (2-[3-(2-hydroxy-1,1-dihydroxymethyl-ethylamino)-propylamino]-2-hydroxymethyl-propane-1,3-diol, Local RMSD=0.10) with 3VR4 (Global RMSD=3.26)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100771
Most Similar Human Ortholog: K7EK77
TM-align score: 0.89 | RMSD: 1.46
Seq Identity: 0.64 | Length: 191 / 551
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000152234 | ATP synthase F1 subunit alpha |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 31 | 21 | 44 |
| disruptive | 3 | 12 | 12 | 26 |
| missense | 3 | 12 | 12 | 19 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 66 | 24 | 41 |
| disruptive | 6 | 43 | 40 | 87 |
| missense | 5 | 28 | 37 | 54 |
PlasmoDB Total SNPs: 124
Non-coding: 103 | Synonymous: 12 | Nonsynonymous: 6 | Stop Codon: 3
Protein Length: 551 | Molecular Weight (kDa): 61.77
UniProt ID(s): O96252
PDB ID(s): None
Isoelectric Point: 8.63
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000194 | ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain |
| InterPro | IPR000793 | ATP synthase, alpha subunit, C-terminal |
| InterPro | IPR004100 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain |
| InterPro | IPR005294 | ATP synthase, F1 complex, alpha subunit |
| InterPro | IPR020003 | ATPase, alpha/beta subunit, nucleotide-binding domain, active site |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR033732 | ATP synthase, F1 complex, alpha subunit nucleotide-binding domain |
| InterPro | IPR036121 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily |
| PFam | PF00006 | ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain |
| PFam | PF00306 | ATP synthase, alpha subunit, C-terminal |
| PFam | PF02874 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain |
| Superfamily | SSF47917 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily |
| Superfamily | SSF50615 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF52540 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21984828 | ATP synthase complex of Plasmodium falciparum: dimeric assembly in mitochondrialmembranes and resistance to genetic disruption. | Nina PB, Morrisey JM, ..., Vaidya AB | 10.1074/jbc.M111.290973 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |