Product Description: replication factor C subunit 2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005663 | DNA replication factor C complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Process | GO:0006281 | DNA repair |
| Process | GO:0006260 | DNA replication |
| Process | GO:0006261 | DNA-dependent DNA replication |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.07 | 0.96 |
| Trophozoite | expressed | 0.63 | 0.64 |
| Schizont | expressed | 0.22 | 0.86 |
| Gametocyte | expressed | 0.44 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0218000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.168 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0314700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0314700)
Modification: Disrupted | RMgm-1814
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96260
"Best" AlphaFill ligand hit: 08T ([[[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-tris(fluoranyl)beryllium, Local RMSD=0.02) with 8UH7 (Global RMSD=7.78)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P06710 | DNA polymerase III subunit tau | Escherichia coli | OrthoMCL | d5-EtITPd5-PhITPd6-NITP |
Ortholog Group (OrthoMCL): OG6_101335
Most Similar Human Ortholog: P35249
TM-align score: 0.89 | RMSD: 2.31
Seq Identity: 0.39 | Length: 323 / 330
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000163918 | replication factor C subunit 4 |
Protein Length: 330 | Molecular Weight (kDa): 37.923
PDB ID(s): None
Isoelectric Point: 6.75
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR008921 | DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal |
| InterPro | IPR013748 | Replication factor C, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF08542 | Replication factor C, C-terminal |
| PFam | PF13177 | |
| Superfamily | SSF48019 | DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 15 | 7 | 20 |
| disruptive | 1 | 11 | 7 | 14 |
| missense | 1 | 11 | 7 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 30 | 13 | 20 |
| disruptive | 2 | 21 | 13 | 59 |
| missense | 2 | 20 | 12 | 44 |
PlasmoDB Total SNPs: 150
Non-coding: 128 | Synonymous: 16 | Nonsynonymous: 4 | Stop Codon: 2
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 33040440 | Plasmodium falciparum replication factor C subunit 1 is involved in genotoxicstress response. | Sheriff O, Yaw A, ..., Preiser PR | 10.1111/cmi.13277 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |