Product Description: rRNA methyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.- (Methyltransferases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0008989 | rRNA (guanine-N1-)-methyltransferase activity |
| Process | GO:0000027 | ribosomal large subunit assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.56 | 0.68 |
| Schizont | possibly expressed | 0.13 | 0.92 |
| Gametocyte | not expressed | 0.41 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0218300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.97 | MFS: -2.069 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0315000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0315000)
Modification: Disrupted | RMgm-1817
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96263
"Best" AlphaFill ligand hit: A2P (adenosine-2'-5'-diphosphate, Local RMSD=0.41) with 3JQP (Global RMSD=14.95)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.- | Ex: homocysteine S-methyltransferase | EDTACTABNickelA9145CtylosintacrineTween 80FTI-2628macrocinadenosineRelomycinphosphate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102128
Most Similar Human Ortholog: Q6IN84
TM-align score: 0.67 | RMSD: 2.50
Seq Identity: 0.15 | Length: 257 / 354
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000274853 | mitochondrial rRNA methyltransferase 1 |
| ENSG00000278619 | mitochondrial rRNA methyltransferase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 23 | 14 | 21 |
| disruptive | 9 | 37 | 25 | 50 |
| missense | 9 | 34 | 25 | 43 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 54 | 11 | 21 |
| disruptive | 17 | 94 | 43 | 106 |
| missense | 13 | 78 | 36 | 73 |
PlasmoDB Total SNPs: 198
Non-coding: 176 | Synonymous: 9 | Nonsynonymous: 12 | Stop Codon: 1
Protein Length: 354 | Molecular Weight (kDa): 42.124
UniProt ID(s): O96263
PDB ID(s): None
Isoelectric Point: 9.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001537 | tRNA/rRNA methyltransferase, SpoU type |
| InterPro | IPR013123 | RNA 2-O ribose methyltransferase, substrate binding |
| InterPro | IPR029028 | Alpha/beta knot methyltransferases |
| InterPro | IPR029064 | 50S ribosomal protein L30e-like |
| PFam | PF00588 | tRNA/rRNA methyltransferase, SpoU type |
| PFam | PF08032 | RNA 2-O ribose methyltransferase, substrate binding |
| Superfamily | SSF55315 | 50S ribosomal protein L30e-like |
| Superfamily | SSF75217 | Alpha/beta knot methyltransferases |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |