About Gene List

PF3D7_0218600

Genome location: Pf3D7_02_v3:755,057..763,428(+)

Genome classification: Core

Function and Localization

Product Description: patatin-like phospholipase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.1.4 (Phospholipase A(2));3.1.1.5 (Lysophospholipase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005737 cytoplasm
Component GO:0005811 lipid droplet
Component GO:0016020 membrane
Function GO:0016298 lipase activity
Function GO:0004806 triglyceride lipase activity
Process GO:0055088 lipid homeostasis
Process GO:0006629 lipid metabolic process
Process GO:0019433 triglyceride catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.53 0.75
Trophozoite expressed 1.26 0.40
Schizont expressed 0.56 0.68
Gametocyte expressed 0.75 0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0218600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 5 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.84 | MFS: -3.005 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96266

"Best" AlphaFill ligand hit: TBR (dodecabromohexatantalum, Local RMSD=0.20) with 2BVL (Global RMSD=21.01)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.1.1.4 phospholipase A2 BRENDA SOAP query unsuccessful
3.1.1.5 lysophospholipase NEMSDSEDTAPCMBEGTAPMSFDMSOCaCl2FeCl3xyleneethanolsulfite...

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
Q8BJ56 Patatin-like phospholipase domain-containing protein 2 OrthoDB CHEMBL3823931CHEMBL4779344CHEMBL4778304CHEMBL4744133...
Q96AD5 Patatin-like phospholipase domain-containing protein 2 OrthoDB CHEMBL3823931

Orthology Information

Ortholog Group (OrthoMCL): OG6_153994

No human ortholog(s)

Protein Information

Protein Length: 2380 | Molecular Weight (kDa): 283.619

UniProt ID(s): O96266

PDB ID(s): None

Isoelectric Point: 9.59

Protein Domain Annotations:

Source Family ID Description
InterPro IPR016035 Acyl transferase/acyl hydrolase/lysophospholipase
InterPro IPR033562 Patatin-like phospholipase domain-containing protein
PFam N/A N/A
Superfamily SSF52151 Acyl transferase/acyl hydrolase/lysophospholipase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 31 137 59 144
disruptive 133 459 196 476
missense 96 363 159 369

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 64 244 93 163
disruptive 247 930 331 803
missense 154 701 243 456

PlasmoDB Total SNPs: 422

Non-coding: 101 | Synonymous: 171 | Nonsynonymous: 148 | Stop Codon: 2

Associated Publications

PMID Title Authors DOI/Link
9804551 Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Tettelin H, ..., Hoffman SL 10.1126/science.282.5391.1126
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
31772212 Functional annotation of serine hydrolases in the asexual erythrocytic stage ofPlasmodium falciparum. Elahi R, Ray WK, ..., Klemba M 10.1038/s41598-019-54009-0