Product Description: pseudouridine synthase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 4.2.1.70 (Pseudouridylate synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0020011 | apicoplast |
| Function | GO:0004730 | pseudouridylate synthase activity |
| Process | GO:0000154 | rRNA modification |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.42 | 0.74 |
| Schizont | expressed | 0.24 | 0.85 |
| Gametocyte | expressed | 0.49 | 0.72 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0219500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.492 | #Insertions: 3
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1443200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0315900)
Modification: Disrupted | RMgm-1822
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96270
"Best" AlphaFill ligand hit: BU1 (1,4-butanediol, Local RMSD=0.03) with 1VIO (Global RMSD=9.95)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.2.1.70 | pseudouridylate synthase | tRNAiodoacetamide5-fluorouracilthiol reagents1,10-phenanthrolinep-chloromercuribenzoate1-(Tetrahydro-2-furanyl)-5-fluorouracil |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 15 | 13 | 16 |
| disruptive | 5 | 25 | 14 | 39 |
| missense | 5 | 24 | 14 | 33 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 38 | 12 | 22 |
| disruptive | 13 | 59 | 28 | 64 |
| missense | 8 | 51 | 26 | 37 |
PlasmoDB Total SNPs: 65
Non-coding: 44 | Synonymous: 16 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 338 | Molecular Weight (kDa): 40.297
UniProt ID(s): O96270
PDB ID(s): None
Isoelectric Point: 10.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000748 | Pseudouridine synthase, RsuA/RluB/E/F |
| InterPro | IPR002942 | RNA-binding S4 domain |
| InterPro | IPR006145 | Pseudouridine synthase, RsuA/RluA |
| InterPro | IPR018496 | Pseudouridine synthase, RsuA/RluB/E/F, conserved site |
| InterPro | IPR020103 | Pseudouridine synthase, catalytic domain superfamily |
| PFam | PF00849 | Pseudouridine synthase, RsuA/RluA |
| PFam | PF01479 | RNA-binding S4 domain |
| Superfamily | SSF55120 | Pseudouridine synthase, catalytic domain superfamily |
| Superfamily | SSF55174 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |