PF3D7_0219600 (RFC1)

Genome location: Pf3D7_02_v3:778,816..782,444(-)

Genome classification: Core

Function and Localization

Product Description: replication factor C subunit 1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005663	DNA replication factor C complex
Component	GO:0034399	nuclear periphery
Component	GO:0005634	nucleus
Function	GO:0003677	DNA binding
Process	GO:0006281	DNA repair
Process	GO:0006260	DNA replication

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.21	0.90
Trophozoite	expressed	0.76	0.58
Schizont	expressed	0.30	0.81
Gametocyte	expressed	0.55	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0219600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.125 | MFS: -2.912 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0316000)

Relative Growth Rate: 0.84 ± 0.74
Confidence: 1.98

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0316000)

Modification: Disrupted | RMgm-1823

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96271

"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.11) with 1SXJ (Global RMSD=6.04)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101059

Most Similar Human Ortholog: P35251

TM-align score: 0.60 | RMSD: 5.93

Seq Identity: 0.27 | Length: 672 / 904

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000035928	replication factor C subunit 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	36	17	33
disruptive	15	54	38	78
missense	11	51	37	72

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	66	23	39
disruptive	25	139	70	129
missense	16	116	58	98

PlasmoDB Total SNPs: 106

Non-coding: 42 | Synonymous: 45 | Nonsynonymous: 18 | Stop Codon: 1

Protein Information

Protein Length: 904 | Molecular Weight (kDa): 104.185

UniProt ID(s): O96271, Q9GQW6

PDB ID(s): None

Isoelectric Point: 9.55

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001357	BRCT domain
InterPro	IPR003593	AAA+ ATPase domain
InterPro	IPR003959	ATPase, AAA-type, core
InterPro	IPR008921	DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal
InterPro	IPR012178	Replication factor C subunit 1
InterPro	IPR013725	DNA replication factor RFC1, C-terminal
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR036420	BRCT domain superfamily
PFam	PF00004	ATPase, AAA-type, core
PFam	PF00533	BRCT domain
PFam	PF08519	DNA replication factor RFC1, C-terminal
Superfamily	SSF48019	DNA polymerase III, clamp loader complex, gamma/delta/delta subunit, C-terminal
Superfamily	SSF52113	BRCT domain superfamily
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
33040440	Plasmodium falciparum replication factor C subunit 1 is involved in genotoxicstress response.	Sheriff O, Yaw A, ..., Preiser PR	10.1111/cmi.13277