Product Description: liver stage antigen 3
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0044228 | host cell surface |
| Component | GO:0020026 | merozoite dense granule |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 3.41 | 0.06 |
| Trophozoite | expressed | 2.20 | 0.20 |
| Schizont | expressed | 1.09 | 0.49 |
| Gametocyte | not expressed | 0.54 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0220000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.002 | #Insertions: 8
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O96275
"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=23.92) with 5A07 (Global RMSD=24.14)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 44 | 12 | 45 |
| disruptive | 84 | 182 | 51 | 213 |
| missense | 63 | 142 | 38 | 150 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 59 | 29 | 61 |
| disruptive | 140 | 265 | 96 | 292 |
| missense | 98 | 202 | 70 | 183 |
PlasmoDB Total SNPs: 609
Non-coding: 116 | Synonymous: 193 | Nonsynonymous: 299 | Stop Codon: 1
Protein Length: 1558 | Molecular Weight (kDa): 175.659
UniProt ID(s): C7DU21, C7DU22, C7DU26, C7DU27, C7DU28, C7DU34, C7DU37, C7DU38, C7DU39, C7DU40, O96275
PDB ID(s): None
Isoelectric Point: 3.86
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9804551 | Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Tettelin H, ..., Hoffman SL | 10.1126/science.282.5391.1126 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368868 | Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14. | Gardner MJ, Shallom SJ, ..., Fraser CM | 10.1038/nature01094 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17828606 | Liver stage antigen 3 isolated from a cDNA library of Plasmodium falciparumerythrocytic stages. | Moyano EM, Gonzalez LM, Arahuetes S, Benito A | 10.1007/s00436-007-0737-y |
| 19139199 | Evidence for multiple B- and T-cell epitopes in Plasmodium falciparum liver-stageantigen 3. | Toure-Balde A, Perlaza BL, ..., Druilhe P | 10.1128/IAI.00780-07 |
| 19874576 | The malaria candidate vaccine liver stage antigen-3 is highly conserved inPlasmodium falciparum isolates from diverse geographical areas. | Prieur E, Druilhe P | 10.1186/1475-2875-8-247 |
| 28378857 | Immunoscreening of Plasmodium falciparum proteins expressed in a wheat germcell-free system reveals a novel malaria vaccine candidate. | Morita M, Takashima E, ..., Tsuboi T | 10.1038/srep46086 |
| 29162636 | Quantitative Proteomic Profiling Reveals Novel Plasmodium falciparum SurfaceAntigens and Possible Vaccine Candidates. | Nilsson Bark SK, Ahmad R, ..., Marti M | 10.1074/mcp.RA117.000076 |
| 27809852 | Serological markers to measure recent changes in malaria at population level inCambodia | Kerkhof K, Sluydts V, ..., Durnez L | 10.1186/s12936-016-1576-z |
| 12731057 | Immunogenicity and protective efficacy of Plasmodium falciparum liver-stage Ag-3in Aotus lemurinus griseimembra monkeys | Perlaza BL, Zapata C, ..., Herrera S | 10.1002/eji.200323339 |
| 18628827 | Genetic immunisation by liver stage antigen 3 protects chimpanzees againstmalaria despite low immune responses | Daubersies P, Ollomo B, ..., Druilhe P | 10.1371/journal.pone.0002659 |
| 22059951 | Assessment of exposure to Plasmodium falciparum transmission in a low endemicityarea by using multiplex fluorescent microsphere-based serological assays | Sarr JB, Orlandi-Pradines E, ..., Remoue F | 10.1186/1756-3305-4-212 |
| 17041140 | Developmental biology of sporozoite-host interactions in Plasmodium falciparummalaria: implications for vaccine design | Garcia JE, Puentes A, Patarroyo ME | 10.1128/CMR.00063-05 |
| 33663492 | Genomic analysis reveals independent evolution of Plasmodium falciparumpopulations in Ethiopia | Abera D, Kibet CK, ..., Golassa L | 10.1186/s12936-021-03660-y |
| 26692284 | Analysis of antibody profiles in symptomatic malaria in three sentinel sites ofIvory Coast by using multiplex, fluorescent, magnetic, bead-based serologicalassay (MAGPIX) | Koffi D, Toure AO, ..., Perraut R | 10.1186/s12936-015-1043-2 |
| 15309314 | Liver stage antigen 3 Plasmodium falciparum peptides specifically interactingwith HepG2 cells | Garcia JE, Curtidor H, ..., Patarroyo ME | 10.1007/s00109-004-0573-9 |
| 22145028 | Pre-clinical assessment of novel multivalent MSP3 malaria vaccine constructs | Bang G, Prieur E, Roussilhon C, Druilhe P | 10.1371/journal.pone.0028165 |
| 34376744 | In silico characterisation of putative Plasmodium falciparum vaccine candidatesin African malaria populations | Ajibola O, Diop MF, ..., Amambua-Ngwa A | 10.1038/s41598-021-95442-4 |
| 16514282 | Usefulness of the recombinant liver stage antigen-3 for an early serodiagnosis ofPlasmodium falciparum infection | Lee HW, Moon SU, ..., Kim TS | 10.3347/kjp.2006.44.1.49 |
| 15271893 | Long-term multiepitopic cytotoxic-T-lymphocyte responses induced in chimpanzeesby combinations of Plasmodium falciparum liver-stage peptides and lipopeptides | BenMohamed L, Thomas A, Druilhe P | 10.1128/IAI.72.8.4376-4384.2004 |
| 21059211 | A multiplex assay for the simultaneous detection of antibodies against 15Plasmodium falciparum and Anopheles gambiae saliva antigens | Ambrosino E, Dumoulin C, ..., Rogier C | 10.1186/1475-2875-9-317 |
| 18792413 | Protection against Plasmodium falciparum challenge induced in Aotus monkeys byliver-stage antigen-3-derived long synthetic peptides | Perlaza BL, Valencia AZ, ..., Druilhe P | 10.1002/eji.200738055 |