PF3D7_0220100

Genome location: Pf3D7_02_v3:803,384..807,811(+)

Genome classification: Core

Function and Localization

Product Description: DnaJ protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.08	0.53
Trophozoite	possibly expressed	0.34	0.81
Schizont	possibly expressed	0.19	0.89
Gametocyte	not expressed	0.44	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0220100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.125 | MFS: -3.131 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1238200)

Relative Growth Rate: 0.78 ± 0.07
Confidence: 6.69

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96276

"Best" AlphaFill ligand hit: LPA (lipoic acid, Local RMSD=16.75) with 5T8U (Global RMSD=21.44)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102152

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	28	12	41
disruptive	41	98	41	116
missense	30	90	37	91

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	53	23	56
disruptive	74	210	85	245
missense	52	167	64	141

PlasmoDB Total SNPs: 139

Non-coding: 50 | Synonymous: 38 | Nonsynonymous: 51 | Stop Codon: 0

Protein Information

Protein Length: 997 | Molecular Weight (kDa): 117.289

UniProt ID(s): O96276

PDB ID(s): None

Isoelectric Point: 8.97

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001623	DnaJ domain
InterPro	IPR018253	DnaJ domain, conserved site
InterPro	IPR019111	Plasmodium RESA, N-terminal
InterPro	IPR026894	DNAJ-containing protein, X-domain
InterPro	IPR036869	Chaperone J-domain superfamily
PFam	PF00226	DnaJ domain
PFam	PF09687	Plasmodium RESA, N-terminal
PFam	PF14308	DNAJ-containing protein, X-domain
Superfamily	SSF46565	Chaperone J-domain superfamily

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
12368868	Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14.	Gardner MJ, Shallom SJ, ..., Fraser CM	10.1038/nature01094
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
31631203	A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum.	Das D, Krishnan SR, Roy A, Bulusu G	10.1039/c9mo00124g