PF3D7_0220800 (CLAG2)

Genome location: Pf3D7_02_v3:838,594..844,668(+)

Genome classification: Core

Function and Localization

Product Description: cytoadherence linked asexual protein 2

SignalP Peptide: MVSSVKSSLFLLIFFLYLKKNVIC

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020008	rhoptry

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.12	0.94
Trophozoite	not expressed	0.11	0.93
Schizont	expressed	2.09	0.27
Gametocyte	not expressed	0.05	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0220800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.04 | #Insertions: 11

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1400600)

Relative Growth Rate: 0.28 ± 0.27
Confidence: 4.02

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1400600)

Modification: Tagged | RMgm-4719

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O96279

"Best" AlphaFill ligand hit: DTP (, Local RMSD=2.69) with 5IM3 (Global RMSD=12.64)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_149547

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	57	27	16	30
disruptive	120	73	36	130
missense	79	61	28	102

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	64	43	22	43
disruptive	160	126	71	254
missense	104	92	56	156

PlasmoDB Total SNPs: 513

Non-coding: 234 | Synonymous: 143 | Nonsynonymous: 135 | Stop Codon: 1

Protein Information

Protein Length: 1440 | Molecular Weight (kDa): 171.161

UniProt ID(s): B0M0V5, B0M153, B0M160, O96279, Q4AE86

PDB ID(s): None

Isoelectric Point: 7.56

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR005553	Cytoadherence-linked asexual protein
PFam	PF03805	Cytoadherence-linked asexual protein
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
9804551	Chromosome 2 sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Tettelin H, ..., Hoffman SL	10.1126/science.282.5391.1126
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
15953647	Apical expression of three RhopH1/Clag proteins as components of the Plasmodiumfalciparum RhopH complex.	Kaneko O, Yim Lim BY, ..., Torii M	https://pubmed.ncbi.nlm.nih.gov/15953647/
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23264726	Stable Allele Frequency Distribution of the Plasmodium falciparum clag GenesEncoding Components of the High Molecular Weight Rhoptry Protein Complex	Alexandre JS, Xangsayarath P, ..., Kaneko O	10.2149/tmh.2012-13
18155305	Diversity and evolution of the rhoph1/clag multigene family of Plasmodiumfalciparum	Iriko H, Kaneko O, ..., Torii M	10.1016/j.molbiopara.2007.11.004
22028613	Positive selection on the Plasmodium falciparum clag2 gene encoding a componentof the erythrocyte-binding rhoptry protein complex	Alexandre JS, Kaewthamasorn M, ..., Kaneko O	10.2149/tmh.2011-12
37653544	Recent increase in low complexity polygenomic infections and sialicacid-independent invasion pathways in Plasmodium falciparum from Western Gambia	Nganyewo NN, Bojang F, ..., Amambua-Ngwa A	10.1186/s13071-023-05929-4