Product Description: acyl-CoA synthetase
SignalP Peptide: MGKVFTIYVFVIYLIYIRPNVS
# Transmembrane Domains: 0
EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0016020 | membrane |
| Function | GO:0016208 | AMP binding |
| Function | GO:0004467 | long-chain fatty acid-CoA ligase activity |
| Process | GO:0006631 | fatty acid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 1.00 |
| Trophozoite | not expressed | 0.00 | 1.00 |
| Schizont | possibly expressed | 0.00 | 1.00 |
| Gametocyte | not expressed | 0.00 | 1.00 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0301000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.121 | MFS: -2.996 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97330
"Best" AlphaFill ligand hit: POP (pyrophosphate 2-, Local RMSD=0.10) with 3LNV (Global RMSD=9.82)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.3 | long-chain-fatty-acid-CoA ligase | ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100152
Most Similar Human Ortholog: B7Z3Z9
TM-align score: 0.90 | RMSD: 2.69
Seq Identity: 0.22 | Length: 515 / 819
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000151726 | acyl-CoA synthetase long chain family member 1 |
| ENSG00000164398 | acyl-CoA synthetase long chain family member 6 |
| ENSG00000197142 | acyl-CoA synthetase long chain family member 5 |
| ENSG00000281938 | novel transcript |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 21 | 9 | 15 |
| disruptive | 27 | 64 | 41 | 78 |
| missense | 23 | 57 | 36 | 62 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 31 | 8 | 22 |
| disruptive | 38 | 130 | 60 | 122 |
| missense | 33 | 110 | 51 | 68 |
PlasmoDB Total SNPs: 76
Non-coding: 0 | Synonymous: 34 | Nonsynonymous: 40 | Stop Codon: 2
Protein Length: 819 | Molecular Weight (kDa): 94.264
UniProt ID(s): O97330
PDB ID(s): None
Isoelectric Point: 9.22
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000873 | AMP-dependent synthetase/ligase |
| InterPro | IPR020845 | AMP-binding, conserved site |
| PFam | PF00501 | AMP-dependent synthetase/ligase |
| Superfamily | SSF56801 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 37653437 | Production of S-methyl-methionine using engineered Saccharomyces cerevisiae sakeK6 | Lee JM, Park MH, Park BS, Oh MK | 10.1093/jimb/kuad026 |
| 36533081 | 2-hydroxyisobutyric acid (2-HIBA) modulates ageing and fat deposition in Caenorhabditis elegans | Schifano E, Conta G, ..., Miccheli A | 10.3389/fmolb.2022.986022 |
| 36358951 | Bio-Multifunctional Sponges Containing Alginate/Chitosan/SargassumPolysaccharides Promote the Healing of Full-Thickness Wounds | Quan W, Li P, ..., Ouyang Q | 10.3390/biom12111601 |
| 37582309 | Preparation of low molecular weight polysaccharides from Tremella fuciformis byultrasonic-assisted H(2)O(2)-Vc method: Structural characteristics, in vivoantioxidant activity and stress resistance | Lee Q, Han X, ..., Zeng F | 10.1016/j.ultsonch.2023.106555 |