Product Description: serine/threonine protein kinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004707 | MAP kinase activity |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0010468 | regulation of gene expression |
| Process | GO:0050684 | regulation of mRNA processing |
| Process | GO:0000245 | spliceosomal complex assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.43 | 0.80 |
| Trophozoite | expressed | 0.69 | 0.62 |
| Schizont | possibly expressed | 0.17 | 0.90 |
| Gametocyte | possibly expressed | 0.90 | 0.54 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0302100
Resistome Missense Mutations: I957V
Resistome Compounds with Missense Mutations: MMV665794
Resistome # Samples with Disruptive Mutations: 8 (3 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.991 | MFS: -2.546 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0401100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0401100)
Modification: Disrupted | RMgm-1829
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77306
"Best" AlphaFill ligand hit: 5XH (~{n}-[1-[(3~{r})-1-[4-(dimethylamino)but-2-enoyl]azepan-3-yl]-7-methyl-benzimidazol-2-yl]-2-methyl-pyridine-4-carboxamide, Local RMSD=0.20) with 6Q2A (Global RMSD=6.20)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q96SB4 | SRSF protein kinase 1 | Homo sapiens | OrthoDB | SPHINXSPHINX scaffold, 1SPHINX scaffold, 2US9695160, SPHINX7... |
| Q9UPE1 | SRSF protein kinase 3 | Homo sapiens | OrthoDB | CHEMBL4077195Staurosporine, 8Staurosporine, 8 |
| O70551 | SRSF protein kinase 1 | OrthoDB | CHEMBL4064277 | |
| P78362 | SRSF protein kinase 2 | Homo sapiens | OrthoDB | US9126931, 366CHEMBL3747406CHEMBL3746395CHEMBL3747611... |
Ortholog Group (OrthoMCL): OG6_100556
Most Similar Human Ortholog: A8MPP7
TM-align score: 0.73 | RMSD: 3.35
Seq Identity: 0.40 | Length: 394 / 1338
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000096063 | SRSF protein kinase 1 |
| ENSG00000135250 | SRSF protein kinase 2 |
| ENSG00000184343 | SRSF protein kinase 3 |
Protein Length: 1338 | Molecular Weight (kDa): 157.372
UniProt ID(s): O77306
PDB ID(s): None
Isoelectric Point: 5.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 61 | 13 | 50 |
| disruptive | 39 | 123 | 74 | 160 |
| missense | 30 | 111 | 62 | 136 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 71 | 27 | 65 |
| disruptive | 76 | 218 | 102 | 226 |
| missense | 49 | 184 | 93 | 162 |
PlasmoDB Total SNPs: 220
Non-coding: 130 | Synonymous: 56 | Nonsynonymous: 34 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 20870716 | PfSRPK1, a novel splicing-related kinase from Plasmodium falciparum. | Dixit A, Singh PK, ..., Sharma P | 10.1074/jbc.M110.119255 |
| 25188378 | Inhibition of the SR protein-phosphorylating CLK kinases of Plasmodium falciparumimpairs blood stage replication and malaria transmission. | Kern S, Agarwal S, ..., Pradel G | 10.1371/journal.pone.0105732 |
| 37204422 | Genomic variation during culture adaptation of genetically complex Plasmodiumfalciparum clinical isolates | Claessens A, Stewart LB, ..., Conway DJ | 10.1099/mgen.0.001009 |
| 37535401 | Plasmodium microtubule-binding protein EB1 is critical for partitioning of nucleiin male gametogenesis | Mauer S, Camargo N, ..., Kumar S | 10.1128/mbio.00822-23 |