Genome location: Pf3D7_03_v3:119,098..124,924(+)
Genome classification: Core
Product Description: cytoadherence linked asexual protein 3.2
SignalP Peptide: MVSFFKTPIIIFFFLLCLNEKVLC
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0005634 | nucleus |
| Component | GO:0020008 | rhoptry |
| Function | GO:0005253 | anion channel activity |
| Function | GO:0050839 | cell adhesion molecule binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.27 | 0.88 |
| Trophozoite | expressed | 0.31 | 0.85 |
| Schizont | expressed | 3.55 | 0.16 |
| Gametocyte | possibly expressed | 0.11 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0302200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.932 | MFS: -2.926 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1400600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77309
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 34 | 5 | 6 | 14 |
| disruptive | 36 | 27 | 7 | 32 |
| missense | 23 | 19 | 7 | 29 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 34 | 14 | 4 | 15 |
| disruptive | 50 | 32 | 17 | 51 |
| missense | 28 | 26 | 12 | 39 |
PlasmoDB Total SNPs: 717
Non-coding: 223 | Synonymous: 266 | Nonsynonymous: 225 | Stop Codon: 3
Protein Length: 1416 | Molecular Weight (kDa): 167.489
UniProt ID(s): A0A1W6IZJ2, A0A1W6IZK1, B0M0V7, B0M152, B0M156, B0M163, O77309, Q4AE82
PDB ID(s): None
Isoelectric Point: 7.14
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005553 | Cytoadherence-linked asexual protein |
| PFam | PF03805 | Cytoadherence-linked asexual protein |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 10737759 | clag9: A cytoadherence gene in Plasmodium falciparum essential for binding ofparasitized erythrocytes to CD36. | Trenholme KR, Gardiner DL, ..., Kemp DJ | 10.1073/pnas.040561197 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15953647 | Apical expression of three RhopH1/Clag proteins as components of the Plasmodiumfalciparum RhopH complex. | Kaneko O, Yim Lim BY, ..., Torii M | https://pubmed.ncbi.nlm.nih.gov/15953647/ |
| 21320181 | Functional analysis of epigenetic regulation of tandem RhopH1/clag genes revealsa role in Plasmodium falciparum growth. | Comeaux CA, Coleman BI, ..., Duraisingh MT | 10.1111/j.1365-2958.2011.07572.x |
| 21620134 | Malaria parasite clag3 genes determine channel-mediated nutrient uptake byinfected red blood cells. | Nguitragool W, Bokhari AA, ..., Desai SA | 10.1016/j.cell.2011.05.002 |
| 24699906 | Proteolysis at a specific extracellular residue implicates integral membraneCLAG3 in malaria parasite nutrient channels. | Nguitragool W, Rayavara K, Desai SA | 10.1371/journal.pone.0093759 |
| 28252383 | Plasmodium falciparum parasites deploy Rhop H2 into the host erythrocyte to obtainnutrients, grow and replicate | Counihan NA, Chisholm SA, ..., de Koning-Ward TF | 10.7554/eLife.23217 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 32069335 | Complex nutrient channel phenotypes despite Mendelian inheritance in a Plasmodiumfalciparum genetic cross. | Gupta A, Bokhari AAB, ..., Desai SA | 10.1371/journal.ppat.1008363 |
| 33774908 | Characterisation of complexes formed by parasite proteins exported into the hostcell compartment of Plasmodium falciparum infected red blood cells. | Jonsdottir TK, Counihan NA, ..., Gilson PR | 10.1111/cmi.13332 |
| 31689662 | RhopH3, rhoptry gene conserved in the free-living alveolate flagellate Colpodellasp. (Apicomplexa) | Sam-Yellowe TY, Yadavalli R, ..., Drazba JA | 10.1016/j.ejop.2019.125637 |
| 21306446 | Heterochromatin formation in bistable chromatin domains controls the epigeneticrepression of clonally variant Plasmodium falciparum genes linked to erythrocyteinvasion | Crowley VM, Rovira-Graells N, Ribas de Pouplana L, Cortes A | 10.1111/j.1365-2958.2011.07574.x |
| 30782998 | Identification of Antimalarial Compounds That Require CLAG3 for Their Uptake byPlasmodium falciparum-Infected Erythrocytes | Mira-Martinez S, Pickford AK, ..., Rosanas-Urgell A | 10.1128/AAC.00052-19 |
| 23819786 | Epigenetic switches in clag3 genes mediate blasticidin S resistance in malariaparasites | Mira-Martinez S, Rovira-Graells N, ..., Cortes A | 10.1111/cmi.12162 |
| 22028613 | Positive selection on the Plasmodium falciparum clag2 gene encoding a componentof the erythrocyte-binding rhoptry protein complex | Alexandre JS, Kaewthamasorn M, ..., Kaneko O | 10.2149/tmh.2011-12 |
| 28419281 | Expression of the Plasmodium falciparum Clonally Variant clag3 Genes in HumanInfections | Mira-Martinez S, van Schuppen E, ..., Cortes A | 10.1093/infdis/jix053 |
| 26202963 | Deciphering the principles that govern mutually exclusive expression ofPlasmodium falciparum clag3 genes | Rovira-Graells N, Crowley VM, ..., Cortes A | 10.1093/nar/gkv730 |
| 23442253 | A Poisson hierarchical modelling approach to detecting copy number variation insequence coverage data | Sepulveda N, Campino SG, ..., Clark TG | 10.1186/1471-2164-14-128 |
| 17676953 | Epigenetic silencing of Plasmodium falciparum genes linked to erythrocyteinvasion | Cortes A, Carret C, ..., Holder AA | 10.1371/journal.ppat.0030107 |