Genome location: Pf3D7_03_v3:134,486..141,185(+)
Genome classification: Core
Product Description: cytoadherence linked asexual protein 3.1
SignalP Peptide: MVSFFKTPIFILIIFLYLNEKVIC
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0005634 | nucleus |
| Component | GO:0020008 | rhoptry |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005253 | anion channel activity |
| Function | GO:0050839 | cell adhesion molecule binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.19 | 0.91 |
| Trophozoite | expressed | 0.16 | 0.92 |
| Schizont | expressed | 1.77 | 0.57 |
| Gametocyte | expressed | 0.09 | 0.95 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0302500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.547 | #Insertions: 2
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1400600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77310
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 29 | 11 | 2 | 9 |
| disruptive | 62 | 22 | 17 | 33 |
| missense | 29 | 18 | 12 | 27 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 33 | 16 | 0 | 11 |
| disruptive | 69 | 41 | 19 | 48 |
| missense | 33 | 31 | 15 | 35 |
PlasmoDB Total SNPs: 698
Non-coding: 210 | Synonymous: 262 | Nonsynonymous: 226 | Stop Codon: 0
Protein Length: 1417 | Molecular Weight (kDa): 167.242
UniProt ID(s): A0A1W6IZJ1, A0A1W6IZJ5, B0M155, B0M162, O77310, Q4AE85
PDB ID(s): None
Isoelectric Point: 7.17
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005553 | Cytoadherence-linked asexual protein |
| PFam | PF03805 | Cytoadherence-linked asexual protein |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 10737759 | clag9: A cytoadherence gene in Plasmodium falciparum essential for binding ofparasitized erythrocytes to CD36. | Trenholme KR, Gardiner DL, ..., Kemp DJ | 10.1073/pnas.040561197 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15953647 | Apical expression of three RhopH1/Clag proteins as components of the Plasmodiumfalciparum RhopH complex. | Kaneko O, Yim Lim BY, ..., Torii M | https://pubmed.ncbi.nlm.nih.gov/15953647/ |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21320181 | Functional analysis of epigenetic regulation of tandem RhopH1/clag genes revealsa role in Plasmodium falciparum growth. | Comeaux CA, Coleman BI, ..., Duraisingh MT | 10.1111/j.1365-2958.2011.07572.x |
| 21620134 | Malaria parasite clag3 genes determine channel-mediated nutrient uptake byinfected red blood cells. | Nguitragool W, Bokhari AA, ..., Desai SA | 10.1016/j.cell.2011.05.002 |
| 23819786 | Epigenetic switches in clag3 genes mediate blasticidin S resistance in malariaparasites. | Mira-Martinez S, Rovira-Graells N, ..., Cortes A | 10.1111/cmi.12162 |
| 27299521 | Malaria Parasite CLAG3, a Protein Linked to Nutrient Channels, Participates inHigh Molecular Weight Membrane-Associated Complexes in the Infected Erythrocyte. | Zainabadi K | 10.1371/journal.pone.0157390 |
| 28252383 | Plasmodium falciparum parasites deploy Rhop H2 into the host erythrocyte to obtainnutrients, grow and replicate | Counihan NA, Chisholm SA, ..., de Koning-Ward TF | 10.7554/eLife.23217 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 29739907 | C LAG3 Self-Associates in Malaria Parasites and Quantitatively Determines Nutrient Uptake Channels at the Host Membrane | Gupta A, Balabaskaran-Nina P, ..., Desai SA | 10.1128/mBio.02293-17 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 32069335 | Complex nutrient channel phenotypes despite Mendelian inheritance in a Plasmodiumfalciparum genetic cross. | Gupta A, Bokhari AAB, ..., Desai SA | 10.1371/journal.ppat.1008363 |
| 32900800 | Live-Cell F RET Reveals that Malaria Nutrient Channel Proteins C LAG3 and Rhop H2Remain Associated throughout Their Tortuous Trafficking | Ahmad M, Manzella-Lapeira J, ..., Desai SA | 10.1128/mBio.01354-20 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 35393572 | RhopH2 and RhopH3 export enables assembly of the RhopH complex on P.falciparum-infected erythrocyte membranes. | Pasternak M, Verhoef JMJ, ..., Cowman AF | 10.1038/s42003-022-03290-3 |
| 28089547 | Antibody profiles to wheat germ cell-free system synthesized Plasmodiumfalciparum proteins correlate with protection from symptomatic malaria in Uganda | Kanoi BN, Takashima E, ..., Tsuboi T | 10.1016/j.vaccine.2017.01.001 |
| 17175193 | The Plasmodium falciparum RhopH2 promoter and first 24 amino acids are sufficientto target proteins to the rhoptries | Ghoneim A, Kaneko O, Tsuboi T, Torii M | 10.1016/j.parint.2006.11.001 |
| 30782998 | Identification of Antimalarial Compounds That Require CLAG3 for Their Uptake byPlasmodium falciparum-Infected Erythrocytes | Mira-Martinez S, Pickford AK, ..., Rosanas-Urgell A | 10.1128/AAC.00052-19 |
| 21306446 | Heterochromatin formation in bistable chromatin domains controls the epigeneticrepression of clonally variant Plasmodium falciparum genes linked to erythrocyteinvasion | Crowley VM, Rovira-Graells N, Ribas de Pouplana L, Cortes A | 10.1111/j.1365-2958.2011.07574.x |
| 22028613 | Positive selection on the Plasmodium falciparum clag2 gene encoding a componentof the erythrocyte-binding rhoptry protein complex | Alexandre JS, Kaewthamasorn M, ..., Kaneko O | 10.2149/tmh.2011-12 |
| 28419281 | Expression of the Plasmodium falciparum Clonally Variant clag3 Genes in HumanInfections | Mira-Martinez S, van Schuppen E, ..., Cortes A | 10.1093/infdis/jix053 |
| 26202963 | Deciphering the principles that govern mutually exclusive expression ofPlasmodium falciparum clag3 genes | Rovira-Graells N, Crowley VM, ..., Cortes A | 10.1093/nar/gkv730 |
| 25573429 | A role for adaptor protein complex 1 in protein targeting to rhoptry organellesin Plasmodium falciparum | Kaderi Kibria KM, Rawat K, ..., Malhotra P | 10.1016/j.bbamcr.2014.12.030 |
| 34446549 | Native structure of the RhopH complex, a key determinant of malaria parasitenutrient acquisition | Ho CM, Jih J, ..., Zhou ZH | 10.1073/pnas.2100514118 |
| 17676953 | Epigenetic silencing of Plasmodium falciparum genes linked to erythrocyteinvasion | Cortes A, Carret C, ..., Holder AA | 10.1371/journal.ppat.0030107 |