Product Description: N-ethylmaleimide-sensitive fusion protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.6 (Vesicle-fusing ATPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005795 | Golgi stack |
| Component | GO:0020020 | food vacuole |
| Component | GO:0030430 | host cell cytoplasm |
| Function | GO:0016887 | ATP hydrolysis activity |
| Process | GO:0043001 | Golgi to plasma membrane protein transport |
| Process | GO:0048211 | Golgi vesicle docking |
| Process | GO:0006888 | endoplasmic reticulum to Golgi vesicle-mediated transport |
| Process | GO:0006891 | intra-Golgi vesicle-mediated transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.09 | 0.95 |
| Trophozoite | expressed | 0.20 | 0.87 |
| Schizont | expressed | 0.12 | 0.91 |
| Gametocyte | expressed | 0.10 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0303000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.186 | MFS: -3.241 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0401600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77313
"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=0.15) with 5C18 (Global RMSD=7.36)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.6 | vesicle-fusing ATPase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101440
Most Similar Human Ortholog: B4DH19
TM-align score: 0.91 | RMSD: 2.74
Seq Identity: 0.45 | Length: 636 / 783
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000073969 | N-ethylmaleimide sensitive factor, vesicle fusing ATPase |
| ENSG00000276262 | N-ethylmaleimide sensitive factor, vesicle fusing ATPase |
| ENSG00000278174 | N-ethylmaleimide sensitive factor, vesicle fusing ATPase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 50 | 20 | 48 |
| disruptive | 12 | 66 | 28 | 110 |
| missense | 8 | 65 | 26 | 103 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 113 | 29 | 42 |
| disruptive | 21 | 144 | 69 | 170 |
| missense | 14 | 129 | 62 | 119 |
PlasmoDB Total SNPs: 58
Non-coding: 16 | Synonymous: 27 | Nonsynonymous: 15 | Stop Codon: 0
Protein Length: 783 | Molecular Weight (kDa): 89.134
UniProt ID(s): O77313
PDB ID(s): None
Isoelectric Point: 6.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR003960 | ATPase, AAA-type, conserved site |
| InterPro | IPR009010 | Aspartate decarboxylase-like domain superfamily |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR029067 | CDC48 domain 2-like superfamily |
| InterPro | IPR039812 | Vesicle-fusing ATPase |
| InterPro | IPR041569 | AAA ATPase, AAA+ lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF17862 | AAA ATPase, AAA+ lid domain |
| Superfamily | SSF50692 | Aspartate decarboxylase-like domain superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF54585 | CDC48 domain 2-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 11278971 | A homologue of N-ethylmaleimide-sensitive factor in the malaria parasitePlasmodium falciparum is exported and localized in vesicular structures in thecytoplasm of infected erythrocytes in the brefeldin A-sensitive pathway. | Hayashi M, Taniguchi S, ..., Moriyama Y | 10.1074/jbc.M011709200 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |