Product Description: DNA-directed RNA polymerases I, II, and III subunit RPABC2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005736 | RNA polymerase I complex |
| Component | GO:0005665 | RNA polymerase II, core complex |
| Component | GO:0005666 | RNA polymerase III complex |
| Function | GO:0003899 | DNA-directed 5'-3' RNA polymerase activity |
| Process | GO:0006366 | transcription by RNA polymerase II |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.09 | 0.95 |
| Trophozoite | expressed | 0.10 | 0.94 |
| Schizont | expressed | 0.02 | 0.99 |
| Gametocyte | possibly expressed | 0.05 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0303300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.147 | MFS: -3.103 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0401900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77315
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101231
Most Similar Human Ortholog: U3KPY1
TM-align score: 0.76 | RMSD: 1.80
Seq Identity: 0.49 | Length: 94 / 153
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100142 | RNA polymerase II, I and III subunit F |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 99
Non-coding: 95 | Synonymous: 3 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 153 | Molecular Weight (kDa): 17.834
UniProt ID(s): O77315
PDB ID(s): None
Isoelectric Point: 3.88
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006110 | RNA polymerase, subunit omega/K/RPB6 |
| InterPro | IPR006111 | Archaeal RpoK/eukaryotic RPB6 RNA polymerase subunit |
| InterPro | IPR020708 | DNA-directed RNA polymerase, 14-18kDa subunit, conserved site |
| InterPro | IPR028363 | DNA-directed RNA polymerase, subunit RPB6 |
| InterPro | IPR036161 | RPB6/omega subunit-like superfamily |
| PFam | PF01192 | RNA polymerase, subunit omega/K/RPB6 |
| Superfamily | SSF63562 | RPB6/omega subunit-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23836886 | Correct assembly of RNA polymerase II depends on the foot domain and is requiredfor multiple steps of transcription in Saccharomyces cerevisiae | Garrido-Godino AI, Garcia-Lopez MC, Navarro F | 10.1128/MCB.00262-13 |
| 16246139 | An in silico analysis of trypanosomatid RNA polymerases: insights into theirunusual transcription | Kelly S, Wickstead B, Gull K | 10.1042/BST0331435 |
| 16621069 | Characterization of RNA polymerase II subunits of Trypanosoma brucei | Devaux S, Lecordier L, ..., Vanhamme L | 10.1016/j.molbiopara.2006.02.020 |
| 11486042 | Partners of Rpb8p, a small subunit shared by yeast RNA polymerases I, II and III | Briand JF, Navarro F, ..., Thuriaux P | 10.1128/MCB.21.17.6056-6065.2001 |
| 16730080 | Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei | Nguyen TN, Schimanski B, ..., Gunzl A | 10.1016/j.molbiopara.2006.02.023 |
| 11918671 | Level of the RNA polymerase II in the fission yeast stays constant butphosphorylation of its carboxyl terminal domain varies depending on the phase andrate of cell growth | Sakurai H, Ishihama A | 10.1046/j.1365-2443.2002.00522.x |
| 11168594 | Transcription organization and mRNA levels of the genes for all 12 subunits ofthe fission yeast RNA polymerase II | Sakurai H, Ishihama A | 10.1046/j.1365-2443.2001.00394.x |
| 27001033 | Rpb1 foot mutations demonstrate a major role of Rpb4 in mRNA stability duringstress situations in yeast | Garrido-Godino AI, Garcia-Lopez MC, ..., Navarro F | 10.1016/j.bbagrm.2016.03.008 |