PF3D7_0303400 (DHHC1)

Genome location: Pf3D7_03_v3:177,446..181,753(+)

Genome classification: Core

Function and Localization

Product Description: palmitoyltransferase DHHC1

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.225 (Protein S-acyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000139	Golgi membrane
Component	GO:0070258	inner membrane pellicle complex
Function	GO:0016409	palmitoyltransferase activity
Process	GO:0018345	protein palmitoylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.99
Trophozoite	expressed	0.09	0.94
Schizont	expressed	0.28	0.81
Gametocyte	possibly expressed	0.14	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0303400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.127 | MFS: -3.224 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O97224

"Best" AlphaFill ligand hit: PKZ (palmitoyl-coa, Local RMSD=0.02) with 7KHM (Global RMSD=6.83)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.-	Ex: [myelin-proteolipid] O-palmitoyltransferase	CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.225	protein S-acyltransferase	stearoyl-CoAmyristoyl-CoA2-Bromopalmitate2-bromo-palmitateacyl-CoA binding protein

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_130937

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	43	16	40
disruptive	6	28	19	43
missense	6	28	19	41

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	62	34	42
disruptive	8	69	40	110
missense	7	61	33	78

PlasmoDB Total SNPs: 235

Non-coding: 204 | Synonymous: 28 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 616 | Molecular Weight (kDa): 73.863

UniProt ID(s): O97224, Q689C7

PDB ID(s): None

Isoelectric Point: 8.23

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001594	Palmitoyltransferase, DHHC domain
InterPro	IPR002110	Ankyrin repeat
InterPro	IPR020683	Ankyrin repeat-containing domain
InterPro	IPR036770	Ankyrin repeat-containing domain superfamily
PFam	PF01529	Palmitoyltransferase, DHHC domain
PFam	PF12796	Ankyrin repeat-containing domain
Superfamily	SSF48403	Ankyrin repeat-containing domain superfamily

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
15882865	Plasmodium falciparum: characterization of a late asexual stage golgi proteincontaining both ankyrin and DHHC domains.	Seydel KB, Gaur D, ..., Miller LH	10.1016/j.exppara.2005.03.030
25425642	The role of palmitoylation for protein recruitment to the inner membrane complexof the malaria parasite.	Wetzel J, Herrmann S, ..., Gilberger TW	10.1074/jbc.M114.598094
23687301	In silico screening for palmitoyl substrates reveals a role for DHHC1/3/10(zDHHC1/3/11)-mediated neurochondrin palmitoylation in its targeting toRab5-positive endosomes	Oku S, Takahashi N, Fukata Y, Fukata M	10.1074/jbc.M112.431676