Genome location: Pf3D7_03_v3:191,168..193,072(-)
Genome classification: Core
Product Description: lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.168 (Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0016407 | acetyltransferase activity |
| Function | GO:0031405 | lipoic acid binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.12 | 0.94 |
| Trophozoite | possibly expressed | 0.82 | 0.55 |
| Schizont | possibly expressed | 0.59 | 0.64 |
| Gametocyte | not expressed | 0.81 | 0.56 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0303700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.576 | MFS: -3.123 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0402300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0402300)
Modification: Disrupted | RMgm-1838
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97227
"Best" AlphaFill ligand hit: CAO (oxidized coenzyme a, Local RMSD=0.10) with 2II3 (Global RMSD=2.13)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101891
Most Similar Human Ortholog: A0A7P0T9W1
TM-align score: 0.73 | RMSD: 2.17
Seq Identity: 0.34 | Length: 235 / 448
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000137992 | dihydrolipoamide branched chain transacylase E2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 20 | 16 | 38 |
| disruptive | 15 | 56 | 18 | 58 |
| missense | 15 | 56 | 17 | 57 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 44 | 29 | 35 |
| disruptive | 31 | 87 | 44 | 122 |
| missense | 30 | 81 | 40 | 86 |
PlasmoDB Total SNPs: 72
Non-coding: 42 | Synonymous: 9 | Nonsynonymous: 21 | Stop Codon: 0
Protein Length: 448 | Molecular Weight (kDa): 50.99
UniProt ID(s): O97227
PDB ID(s): None
Isoelectric Point: 5.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000089 | Biotin/lipoyl attachment |
| InterPro | IPR001078 | 2-oxoacid dehydrogenase acyltransferase, catalytic domain |
| InterPro | IPR003016 | 2-oxo acid dehydrogenase, lipoyl-binding site |
| InterPro | IPR011053 | Single hybrid motif |
| PFam | PF00198 | 2-oxoacid dehydrogenase acyltransferase, catalytic domain |
| PFam | PF00364 | Biotin/lipoyl attachment |
| Superfamily | SSF51230 | Single hybrid motif |
| Superfamily | SSF52777 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 25116855 | Redox-dependent lipoylation of mitochondrial proteins in Plasmodium falciparum. | Afanador GA, Matthews KA, ..., Prigge ST | 10.1111/mmi.12753 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |