PF3D7_0303800

Genome location: Pf3D7_03_v3:193,499..196,492(+)

Genome classification: Core

Function and Localization

Product Description: IBR domain protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Component	GO:0000151	ubiquitin ligase complex
Function	GO:0031624	ubiquitin conjugating enzyme binding
Function	GO:0061630	ubiquitin protein ligase activity
Process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Process	GO:0000209	protein polyubiquitination
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.04	0.98
Trophozoite	expressed	0.29	0.82
Schizont	expressed	0.22	0.85
Gametocyte	expressed	0.39	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0303800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.96 | MFS: -2.875 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0402400)

Relative Growth Rate: 0.96 ± 0.16
Confidence: 5.00

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0402400)

Modification: Disrupted | RMgm-1839

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O97226

"Best" AlphaFill ligand hit: TMO (trimethylamine oxide, Local RMSD=0.72) with 5N2W (Global RMSD=17.94)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O60260	E3 ubiquitin-protein ligase parkin		OrthoDB	US11401255, Compound # 1US11401255, Compound # 2US11401255, Compound # 3US11401255, Compound # 4...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100963

Most Similar Human Ortholog: Q9Y4X5

TM-align score: 0.62 | RMSD: 5.26

Seq Identity: 0.21 | Length: 438 / 593

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000166233	ariadne RBR E3 ubiquitin protein ligase 1

Protein Information

Protein Length: 593 | Molecular Weight (kDa): 71.873

UniProt ID(s): O97226

PDB ID(s): None

Isoelectric Point: 8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002867	IBR domain
InterPro	IPR031127	E3 ubiquitin ligase RBR family
PFam	PF01485	IBR domain
Superfamily	SSF57850	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	22	13	36
disruptive	16	42	29	76
missense	13	41	26	71

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	41	28	43
disruptive	29	95	48	117
missense	19	85	43	87

PlasmoDB Total SNPs: 109

Non-coding: 70 | Synonymous: 17 | Nonsynonymous: 22 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108