PF3D7_0304000 (IMC1a)

Genome location: Pf3D7_03_v3:198,542..201,584(-)

Genome classification: Core

Function and Localization

Product Description: inner membrane complex protein 1a, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0070258	inner membrane pellicle complex
Process	GO:0044409	entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.01	0.99
Trophozoite	not expressed	0.03	0.98
Schizont	not expressed	0.11	0.93
Gametocyte	not expressed	0.05	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0304000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.253 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0907100)

Relative Growth Rate: 0.99 ± 0.17
Confidence: 4.92

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0402600)

Modification: Mutated | RMgm-1600

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O97228

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_134714

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	54	24	48
disruptive	17	93	45	107
missense	17	84	45	95

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	88	34	54
disruptive	33	158	84	163
missense	31	141	76	119

PlasmoDB Total SNPs: 130

Non-coding: 67 | Synonymous: 39 | Nonsynonymous: 24 | Stop Codon: 0

Protein Information

Protein Length: 861 | Molecular Weight (kDa): 100.847

UniProt ID(s): O97228

PDB ID(s): None

Isoelectric Point: 5.15

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR022086	Inner membrane complex protein
PFam	PF12314	Inner membrane complex protein
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
15533999	A malaria membrane skeletal protein is essential for normal morphogenesis,motility, and infectivity of sporozoites.	Khater EI, Sinden RE, Dessens JT	10.1083/jcb.200406068
27693349	The Plasmodium alveolin IMC1a is stabilised by its terminal cysteine motifs andfacilitates sporozoite morphogenesis and infectivity in a dose-dependent manner.	Al-Khattaf FS, Tremp AZ, El-Houderi A, Dessens JT	10.1016/j.molbiopara.2016.09.004
18650444	IMC1b is a putative membrane skeleton protein involved in cell shape, mechanicalstrength, motility, and infectivity of malaria ookinetes	Tremp AZ, Khater EI, Dessens JT	10.1074/jbc.M801302200
22844474	The alveolin IMC1h is required for normal ookinete and sporozoite motilitybehaviour and host colonisation in Plasmodium berghei	Volkmann K, Pfander C, ..., Brochet M	10.1371/journal.pone.0041409
21098480	Malaria IMC1 membrane skeleton proteins operate autonomously and participate inmotility independently of cell shape	Tremp AZ, Dessens JT	10.1074/jbc.M110.187195