Product Description: EH domain-containing protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0043231 | intracellular membrane-bounded organelle |
| Component | GO:0016020 | membrane |
| Component | GO:0031982 | vesicle |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0006897 | endocytosis |
| Process | GO:0016197 | endosomal transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.15 | 0.48 |
| Trophozoite | expressed | 0.64 | 0.64 |
| Schizont | expressed | 0.79 | 0.56 |
| Gametocyte | expressed | 0.55 | 0.69 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0304200
Resistome Missense Mutations: D218Y
Resistome Compounds with Missense Mutations: MMV688533
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.73 | MFS: 0.766 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0402800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0402800)
Modification: Disrupted | RMgm-1840
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9NLB8
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.44) with 5MVF (Global RMSD=4.94)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100993
Most Similar Human Ortholog: C9JDQ8
TM-align score: 0.95 | RMSD: 1.24
Seq Identity: 0.62 | Length: 161 / 533
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000013016 | EH domain containing 3 |
| ENSG00000024422 | EH domain containing 2 |
| ENSG00000103966 | EH domain containing 4 |
| ENSG00000110047 | EH domain containing 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 21 | 39 | 30 |
| disruptive | 1 | 2 | 9 | 23 |
| missense | 1 | 2 | 8 | 21 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 70 | 25 | 38 |
| disruptive | 3 | 32 | 28 | 109 |
| missense | 2 | 19 | 25 | 81 |
PlasmoDB Total SNPs: 93
Non-coding: 72 | Synonymous: 13 | Nonsynonymous: 6 | Stop Codon: 2
Protein Length: 533 | Molecular Weight (kDa): 62.303
PDB ID(s): None
Isoelectric Point: 8.08
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000261 | EH domain |
| InterPro | IPR011992 | EF-hand domain pair |
| InterPro | IPR022812 | Dynamin superfamily |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR029952 | EH domain-containing protein 4 |
| InterPro | IPR031692 | EH domain-containing protein, N-terminal |
| InterPro | IPR040990 | Domain of unknown function DUF5600 |
| PFam | PF00350 | Dynamin superfamily |
| PFam | PF12763 | EH domain |
| PFam | PF16880 | EH domain-containing protein, N-terminal |
| PFam | PF18150 | Domain of unknown function DUF5600 |
| Superfamily | SSF47473 | EF-hand domain pair |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 26284889 | Eps15 homology domain containing protein of Plasmodium falciparum (PfEHD)associates with endocytosis and vesicular trafficking towards neutral lipidstorage site. | Thakur V, Asad M, ..., Mohmmed A | 10.1016/j.bbamcr.2015.08.007 |
| 32098816 | The Plasmodium falciparum Artemisinin Susceptibility-Associated A P-2 Adaptin mu Subunit is Clathrin Independent and Essential for Schizont Maturation | Henrici RC, Edwards RL, ..., Sutherland CJ | 10.1128/mBio.02918-19 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |