Product Description: DNA-(apurinic or apyrimidinic site) endonuclease
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.-.- (Acting on ester bonds.);4.2.99.18 (DNA-(apurinic or apyrimidinic site) lyase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0008408 | 3'-5' exonuclease activity |
| Function | GO:0003906 | DNA-(apurinic or apyrimidinic site) endonuclease activity |
| Function | GO:0008311 | double-stranded DNA 3'-5' exodeoxyribonuclease activity |
| Function | GO:0016791 | phosphatase activity |
| Function | GO:0004528 | phosphodiesterase I activity |
| Function | GO:0004540 | ribonuclease activity |
| Process | GO:0006281 | DNA repair |
| Process | GO:0006284 | base-excision repair |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.11 | 0.95 |
| Trophozoite | expressed | 0.92 | 0.53 |
| Schizont | expressed | 0.91 | 0.55 |
| Gametocyte | expressed | 1.23 | 0.41 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0305600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.166 | MFS: -2.904 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0404100)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0404100)
Modification: Disrupted | RMgm-4986
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97240
"Best" AlphaFill ligand hit: PGA (2-phosphoglycolic acid, Local RMSD=3.99) with 5WN2 (Global RMSD=4.79)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.-.- | Ex: 5-phospho-D-xylono-1,4-lactonase | paraoxonatropineethylene terephthalatediisopropyl fluorophosphonatebis(2-hydroxyethyl) terephthalic acidphosphoric acid-bis-4-nitrophenyl ester |
| 4.2.99.18 | DNA-(apurinic or apyrimidinic site) lyase | ATPAMPKClCMPUMPP53EDTAdCMPdTMPdAMPdGMPATCA... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 32 | 20 | 39 |
| disruptive | 20 | 57 | 43 | 89 |
| missense | 13 | 52 | 38 | 85 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 69 | 27 | 36 |
| disruptive | 30 | 146 | 71 | 152 |
| missense | 21 | 123 | 62 | 121 |
PlasmoDB Total SNPs: 132
Non-coding: 75 | Synonymous: 35 | Nonsynonymous: 21 | Stop Codon: 1
Protein Length: 617 | Molecular Weight (kDa): 72.443
UniProt ID(s): O97240
PDB ID(s): None
Isoelectric Point: 8.66
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004808 | AP endonuclease 1 |
| InterPro | IPR005135 | Endonuclease/exonuclease/phosphatase |
| InterPro | IPR036691 | Endonuclease/exonuclease/phosphatase superfamily |
| PFam | PF03372 | Endonuclease/exonuclease/phosphatase |
| Superfamily | SSF56219 | Endonuclease/exonuclease/phosphatase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 29914511 | Identification and characterization of DNA endonucleases in Plasmodium falciparum3D7 clone. | Jiang N, Tu Z, ..., Chen Q | 10.1186/s12936-018-2388-0 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33743509 | Plasmodium Ape1 is a multifunctional enzyme in mitochondrial base excision repairand is required for efficient transition from liver to blood stage infection. | Verma N, Shukla H, ..., Habib S | 10.1016/j.dnarep.2021.103098 |
| 38436697 | Apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1) promotes stress granuleformation via YBX1 phosphorylation in ovarian cancer | Mao S, Xie C, ..., Xu H | 10.1007/s00018-023-05086-y |
| 38244516 | Activation of APE1 modulates Nrf2 protected against acute liver injury by inhibithepatocyte ferroptosis and promote hepatocyte autophagy | Diao J, Fan H, ..., Wen X | 10.1016/j.intimp.2024.111529 |
| 38280781 | Leveraging CRISPR/Cas12 signal amplifier to sensitive detection of apurinic/apyrimidinic endonuclease 1 and high-throughput inhibitor screening | Ding S, Yuan Y, ..., Tang Z | 10.1016/j.aca.2024.342212 |
| 38246354 | Methionine sulfoxide reductase 2 regulates Cvt autophagic pathway by altering thestability of Atg19 and Ape1 in Saccharomyces cerevisiae | Chatterjee A, Sepuri NBV | 10.1016/j.jbc.2024.105662 |