PF3D7_0305800

Genome location: Pf3D7_03_v3:281,916..285,260(+)

Genome classification: Core

Function and Localization

Product Description: P-loop containing nucleoside triphosphate hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.4.3 (Adenylate kinase);2.7.4.6 (Nucleoside-diphosphate kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005759	mitochondrial matrix
Function	GO:0004017	adenylate kinase activity
Function	GO:0046899	nucleoside triphosphate adenylate kinase activity
Process	GO:0046033	AMP metabolic process
Process	GO:0009142	nucleoside triphosphate biosynthetic process
Process	GO:0006163	purine nucleotide metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.04	0.98
Trophozoite	not expressed	0.06	0.96
Schizont	not expressed	0.07	0.96
Gametocyte	expressed	0.64	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0305800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.524 | #Insertions: 5

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0404300)

Relative Growth Rate: 0.90 ± 0.16
Confidence: 5.04

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0404300)

Modification: Disrupted | RMgm-1848

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O97242

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.55) with 2CDN (Global RMSD=5.26)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.4.3	adenylate kinase	BRENDA SOAP query unsuccessful
2.7.4.6	nucleoside-diphosphate kinase	ATPUDPAMPGDPGMPEDTAcAMPcGMPdTDPDTNBdCDPMgADP-...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104110

Most Similar Human Ortholog: Q96M32

TM-align score: 0.65 | RMSD: 4.00

Seq Identity: 0.22 | Length: 542 / 1114

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000140057	adenylate kinase 7

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	20	60	37	66
disruptive	47	179	117	207
missense	30	149	108	174

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	27	137	49	69
disruptive	81	376	148	315
missense	52	300	126	190

PlasmoDB Total SNPs: 204

Non-coding: 0 | Synonymous: 95 | Nonsynonymous: 109 | Stop Codon: 0

Protein Information

Protein Length: 1114 | Molecular Weight (kDa): 132.198

UniProt ID(s): O97242

PDB ID(s): None

Isoelectric Point: 4.84

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000850	Adenylate kinase/UMP-CMP kinase
InterPro	IPR007858	Dpy-30 motif
InterPro	IPR026867	Adenylate kinase 7
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR036291	NAD(P)-binding domain superfamily
PFam	PF05186	Dpy-30 motif
Superfamily	SSF51735	NAD(P)-binding domain superfamily
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095