About Gene List

PF3D7_0306300 (GRX1)

Genome location: Pf3D7_03_v3:294,566..297,773(-)

Genome classification: Core

Function and Localization

Product Description: glutaredoxin 1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.8.4.2 (Protein-disulfide reductase (glutathione))

Curated GO (PlasmoDB):

Type GO Term Name
Function GO:0016209 antioxidant activity
Function GO:0019153 protein-disulfide reductase (glutathione) activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 0.27 0.87
Trophozoite expressed 0.93 0.51
Schizont expressed 0.11 0.93
Gametocyte expressed 0.48 0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0306300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.134 | MFS: -3.137 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0404700)

  • Relative Growth Rate: 0.98 ± 0.09
  • Confidence: 6.29

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0404700)

Modification: Disrupted | RMgm-1851

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q9NLB2

"Best" AlphaFill ligand hit: MPO (3[n-morpholino]propane sulfonic acid, Local RMSD=0.24) with 4HJM (Global RMSD=0.40)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
1.8.4.2 protein-disulfide reductase (glutathione) EDTAGSSGcysteinearseniteglucagonOxytoxinAprotininbacitraciniodoacetateVasopressinTriton X-100deoxycholate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100315

Most Similar Human Ortholog: P35754

TM-align score: 0.89 | RMSD: 1.69

Seq Identity: 0.37 | Length: 105 / 111

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000023572 glutaredoxin 2
ENSG00000173221 glutaredoxin

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 0 0 0 1
disruptive 0 0 0 0
missense 0 0 0 0

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 0 0 0 1
disruptive 0 0 0 0
missense 0 0 0 0

PlasmoDB Total SNPs: 323

Non-coding: 317 | Synonymous: 3 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 111 | Molecular Weight (kDa): 12.418

UniProt ID(s): Q7KXR4, Q9NLB2

PDB ID(s): 4HJM, 4MZB, 4MZC, 4N0Z, 4N10, 4N11, 7DIK, 7DIM, 7DIN, 7DIO, 7DIP, 7DIQ, 7DIR, 7DIS, 7DIT, 7DIU, 7DIV, 7DIW, 7DIZ, 7DJ0

Isoelectric Point: 7.74

Protein Domain Annotations:

Source Family ID Description
InterPro IPR002109 Glutaredoxin
InterPro IPR011767 Glutaredoxin active site
InterPro IPR011899 Glutaredoxin, eukaryotic/virial
InterPro IPR014025 Glutaredoxin subgroup
InterPro IPR036249 Thioredoxin-like superfamily
PFam PF00462 Glutaredoxin
Superfamily SSF52833 Thioredoxin-like superfamily

Associated Publications

PMID Title Authors DOI/Link
10448855 The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Bowman S, Lawson D, ..., Barrell BG 10.1038/22964
11479312 Plasmodium falciparum possesses a classical glutaredoxin and a second,glutaredoxin-like protein with a PICOT homology domain. Rahlfs S, Fischer M, Becker K 10.1074/jbc.M105524200
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
20006670 Conformational stability and energetics of Plasmodium falciparum glutaredoxin. Tripathi T, Roseler A, ..., Bhakuni V 10.1016/j.biochi.2009.12.003
24419382 Atomic resolution crystal structure of glutaredoxin 1 from Plasmodium falciparumand comparison with other glutaredoxins. Yogavel M, Tripathi T, ..., Sharma A 10.1107/S1399004713025285
37277068 The impact of glutaredoxin 1 and glutaredoxin 2 double knockout on lensepithelial cell function Zhang J, Yu Y, ..., Wu H 10.1016/j.exer.2023.109521
38243922 Implication of Thioredoxin 1 and Glutaredoxin 1 in H2O2-induced Phosphorylationof JNK and p38 MAP Kinases Poulios E, Roupaka V, ..., Spyrou G 10.2174/0115665240271103231127072635
37115746 The antioxidant protein glutaredoxin 1 is essential for oxidative stress responseand pathogenicity of Toxoplasma gondii Li TT, Zhao DY, ..., Wang JL 10.1096/fj.202201275R