Product Description: end-binding protein 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005881 | cytoplasmic microtubule |
| Component | GO:0005874 | microtubule |
| Component | GO:0005815 | microtubule organizing center |
| Component | GO:0035371 | microtubule plus-end |
| Component | GO:0005880 | nuclear microtubule |
| Component | GO:0005654 | nucleoplasm |
| Component | GO:0051233 | spindle midzone |
| Function | GO:0008017 | microtubule binding |
| Function | GO:0051010 | microtubule plus-end binding |
| Process | GO:0035372 | protein localization to microtubule |
| Process | GO:1904825 | protein localization to microtubule plus-end |
| Process | GO:0031110 | regulation of microtubule polymerization or depolymerization |
| Process | GO:0051225 | spindle assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.08 | 0.96 |
| Trophozoite | not expressed | 0.04 | 0.98 |
| Schizont | not expressed | 0.09 | 0.94 |
| Gametocyte | expressed | 0.66 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0307300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.844 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0405600)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0405600)
Modification: Tagged | RMgm-5329
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O97251
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100898
Most Similar Human Ortholog: C9JB30
TM-align score: 0.60 | RMSD: 2.15
Seq Identity: 0.42 | Length: 143 / 510
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000084764 | microtubule associated protein RP/EB family member 3 |
| ENSG00000101367 | microtubule associated protein RP/EB family member 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 20 | 24 | 29 |
| disruptive | 33 | 49 | 29 | 95 |
| missense | 17 | 45 | 26 | 81 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 53 | 21 | 42 |
| disruptive | 41 | 131 | 67 | 158 |
| missense | 22 | 107 | 58 | 112 |
PlasmoDB Total SNPs: 46
Non-coding: 0 | Synonymous: 24 | Nonsynonymous: 22 | Stop Codon: 0
Protein Length: 510 | Molecular Weight (kDa): 59.544
UniProt ID(s): O97251
PDB ID(s): None
Isoelectric Point: 8.93
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001715 | Calponin homology domain |
| InterPro | IPR004953 | EB1, C-terminal |
| InterPro | IPR027328 | Microtubule-associated protein RP/EB |
| InterPro | IPR036133 | EB1, C-terminal domain superfamily |
| InterPro | IPR036872 | CH domain superfamily |
| PFam | PF00307 | Calponin homology domain |
| PFam | PF03271 | EB1, C-terminal |
| Superfamily | SSF140612 | EB1, C-terminal domain superfamily |
| Superfamily | SSF47576 | CH domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 26466679 | Compartmentalized Toxoplasma EB1 bundles spindle microtubules to secure accuratechromosome segregation. | Chen CT, Kelly M, ..., Gubbels MJ | 10.1091/mbc.E15-06-0437 |
| 36030238 | Repurposing the mitotic machinery to drive cellular elongation and chromatinreorganisation in Plasmodium falciparum gametocytes. | Li J, Shami GJ, ..., Tilley L | 10.1038/s41467-022-32579-4 |
| 37704606 | Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosomesegregation in sexual transmission stages | Zeeshan M, Rea E, ..., Tewari R | 10.1038/s41467-023-41395-3 |
| 37535401 | Plasmodium microtubule-binding protein EB1 is critical for partitioning of nucleiin male gametogenesis | Mauer S, Camargo N, ..., Kumar S | 10.1128/mbio.00822-23 |