About Gene List

PF3D7_0307700 (AKIT10)

Genome location: Pf3D7_03_v3:328,477..336,120(-)

Genome classification: Core

Function and Localization

Product Description: apicomplexan kinetochore protein 10, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0000776 kinetochore
Component GO:0005634 nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.18 0.91
Trophozoite expressed 1.21 0.42
Schizont expressed 1.60 0.30
Gametocyte expressed 1.32 0.37

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0307700

Resistome Missense Mutations: D1495N, Q1496H

Resistome Compounds with Missense Mutations: TCMDC-125334

Resistome # Samples with Disruptive Mutations: 3 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.776 | MFS: -2.472 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0406000)

  • Relative Growth Rate: 0.23 ± 0.16
  • Confidence: 5.00

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0406000)

Modification: Tagged | RMgm-5122

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O97255

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P01042 Kininogen-1 Homo sapiens OrthoMCL BLAST US8592426, 1US8592426, 2US8592426, 3US8592426, 4...

Orthology Information

Ortholog Group (OrthoMCL): OG6_532878

No human ortholog(s)

Protein Information

Protein Length: 1826 | Molecular Weight (kDa): 216.259

UniProt ID(s): O97255, Q689C5

PDB ID(s): None

Isoelectric Point: 6.58

Protein Domain Annotations:

Source Family ID Description
InterPro N/A N/A
PFam N/A N/A
Superfamily SSF141571 N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 21 78 40 103
disruptive 77 219 117 265
missense 43 161 93 214

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 38 150 69 112
disruptive 123 402 208 408
missense 62 309 167 298

PlasmoDB Total SNPs: 537

Non-coding: 260 | Synonymous: 144 | Nonsynonymous: 133 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
10448855 The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Bowman S, Lawson D, ..., Barrell BG 10.1038/22964
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
36006241 Composition and organization of kinetochores show plasticity in apicomplexanchromosome segregation Brusini L, Dos Santos Pacheco N, ..., Brochet M 10.1083/jcb.202111084