Product Description: pre-mRNA-processing factor 19, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.2.27 (RING-type E3 ubiquitin transferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000974 | Prp19 complex |
| Component | GO:0071006 | U2-type catalytic step 1 spliceosome |
| Component | GO:0071013 | catalytic step 2 spliceosome |
| Component | GO:0016363 | nuclear matrix |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Function | GO:0004842 | ubiquitin-protein transferase activity |
| Process | GO:0000349 | generation of catalytic spliceosome for first transesterification step |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.01 | 0.59 |
| Trophozoite | expressed | 0.74 | 0.60 |
| Schizont | expressed | 0.30 | 0.83 |
| Gametocyte | possibly expressed | 0.35 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0308600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.145 | MFS: -2.928 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0406800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77325
"Best" AlphaFill ligand hit: FAR (farnesyl, Local RMSD=1.40) with 1A0R (Global RMSD=3.26)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.2.27 | RING-type E3 ubiquitin transferase | EDTADTPAMLN4924RAPTA-CRAPTA-TRAPTA-EA1 |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P32523 | Pre-mRNA-processing factor 19 | OrthoDB, OrthoMCL, OrthoMCL BLAST | cid_3392731CHEMBL501616CHEMBL514739CHEMBL457440... |
Ortholog Group (OrthoMCL): OG6_102145
Most Similar Human Ortholog: Q9UMS4
TM-align score: 0.65 | RMSD: 1.74
Seq Identity: 0.34 | Length: 340 / 532
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000110107 | pre-mRNA processing factor 19 |
Protein Length: 532 | Molecular Weight (kDa): 60.754
UniProt ID(s): O77325
PDB ID(s): None
Isoelectric Point: 6.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001680 | WD40 repeat |
| InterPro | IPR003613 | U box domain |
| InterPro | IPR013915 | Pre-mRNA-splicing factor 19 |
| InterPro | IPR019775 | WD40 repeat, conserved site |
| InterPro | IPR020472 | G-protein beta WD-40 repeat |
| InterPro | IPR036126 | Tubulin binding cofactor A superfamily |
| InterPro | IPR036322 | WD40-repeat-containing domain superfamily |
| InterPro | IPR038959 | Pre-mRNA-processing factor 19 |
| PFam | PF00400 | WD40 repeat |
| PFam | PF08606 | Pre-mRNA-splicing factor 19 |
| Superfamily | SSF46988 | Tubulin binding cofactor A superfamily |
| Superfamily | SSF50978 | WD40-repeat-containing domain superfamily |
| Superfamily | SSF57850 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 32 | 20 | 30 |
| disruptive | 5 | 44 | 25 | 60 |
| missense | 4 | 37 | 23 | 52 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 58 | 21 | 32 |
| disruptive | 15 | 88 | 61 | 91 |
| missense | 8 | 74 | 50 | 66 |
PlasmoDB Total SNPs: 110
Non-coding: 80 | Synonymous: 20 | Nonsynonymous: 10 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21530591 | A densely overlapping gene fragmentation approach improves yeast two-hybridscreens for Plasmodium falciparum proteins. | Brown HF, Wang L, ..., LaCount DJ | 10.1016/j.molbiopara.2011.04.005 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 37903258 | Human proteins curing yeast prions | Wu S, Edskes HK, Wickner RB | 10.1073/pnas.2314781120 |
| 34850936 | A distinct complex of PRP19-related and trypanosomatid-specific proteins isrequired for pre-mRNA splicing in trypanosomes | Srivastava A, Ambrosio DL, ..., Gunzl A | 10.1093/nar/gkab1152 |