Product Description: dual specificity protein phosphatase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase);3.1.3.48 (Protein-tyrosine-phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0016791 | phosphatase activity |
| Function | GO:0005515 | protein binding |
| Function | GO:0008138 | protein tyrosine/serine/threonine phosphatase activity |
| Function | GO:0017056 | structural constituent of nuclear pore |
| Function | GO:0008270 | zinc ion binding |
| Process | GO:0046823 | negative regulation of nucleocytoplasmic transport |
| Process | GO:0006470 | protein dephosphorylation |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0060284 | regulation of cell development |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.24 | 0.88 |
| Trophozoite | expressed | 0.31 | 0.81 |
| Schizont | expressed | 0.08 | 0.95 |
| Gametocyte | expressed | 0.31 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0309000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.13 | MFS: -2.824 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0407200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0407200)
Modification: Disrupted | RMgm-1868
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77334
"Best" AlphaFill ligand hit: 4NP (4-nitrophenyl phosphate, Local RMSD=0.42) with 4WOH (Global RMSD=0.90)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101936
Most Similar Human Ortholog: V9GYV5
TM-align score: 0.77 | RMSD: 2.25
Seq Identity: 0.17 | Length: 80 / 575
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000081721 | dual specificity phosphatase 12 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 28 | 15 | 37 |
| disruptive | 19 | 65 | 38 | 102 |
| missense | 8 | 47 | 32 | 87 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 54 | 32 | 35 |
| disruptive | 38 | 153 | 79 | 181 |
| missense | 15 | 110 | 69 | 122 |
PlasmoDB Total SNPs: 276
Non-coding: 210 | Synonymous: 47 | Nonsynonymous: 19 | Stop Codon: 0
Protein Length: 575 | Molecular Weight (kDa): 67.787
PDB ID(s): None
Isoelectric Point: 8.3
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000340 | Dual specificity phosphatase, catalytic domain |
| InterPro | IPR016130 | Protein-tyrosine phosphatase, active site |
| InterPro | IPR020422 | Dual specificity protein phosphatase domain |
| InterPro | IPR029021 | Protein-tyrosine phosphatase-like |
| PFam | PF00782 | Dual specificity phosphatase, catalytic domain |
| Superfamily | SSF52799 | Protein-tyrosine phosphatase-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 14698441 | A zinc-binding dual-specificity YVH1 phosphatase in the malaria parasite,Plasmodium falciparum, and its interaction with the nuclear protein, pescadillo. | Kumar R, Musiyenko A, ..., Barik S | https://pubmed.ncbi.nlm.nih.gov/14698441/ |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 36009873 | Mutational Analyses of the Cysteine-Rich Domain of Yvh1, a Protein Required forTranslational Competency in Yeast | Zang H, Shackelford R, Bewley A, Beeser AE | 10.3390/biology11081246 |
| 25736922 | Identification of putative negative regulators of yeast signaling through ascreening for protein phosphatases acting on cell wall integrity and mating MAPKpathways | Sacristan-Reviriego A, Martin H, Molina M | 10.1016/j.fgb.2015.02.011 |
| 32813277 | MAPK pathway-related tyrosine phosphatases regulate development, secondarymetabolism and pathogenicity in fungus Aspergillus flavus | Yang G, Cao X, ..., Wang S | 10.1111/1462-2920.15202 |
| 21474464 | Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNAdecay, glycogen accumulation, and the expression of early meiotic genes inSaccharomyces cerevisiae | Sugiyama M, Nugroho S, ..., Harashima S | 10.1093/jb/mvr040 |