Product Description: aurora-related kinase ARK2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0032133 | chromosome passenger complex |
| Component | GO:0000776 | kinetochore |
| Component | GO:0072686 | mitotic spindle |
| Component | GO:0005876 | spindle microtubule |
| Component | GO:0051233 | spindle midzone |
| Component | GO:0031616 | spindle pole centrosome |
| Function | GO:0035174 | histone serine kinase activity |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0007052 | mitotic spindle organization |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0032465 | regulation of cytokinesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.31 | 0.85 |
| Trophozoite | expressed | 0.88 | 0.55 |
| Schizont | expressed | 0.74 | 0.62 |
| Gametocyte | expressed | 1.63 | 0.28 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0309200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.199 | MFS: -2.77 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0407400)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0407400)
Modification: Tagged | RMgm-5326
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77328
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.29) with 2WQE (Global RMSD=1.73)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100573
Most Similar Human Ortholog: J3QLN8
TM-align score: 0.88 | RMSD: 1.72
Seq Identity: 0.41 | Length: 212 / 1650
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000087586 | aurora kinase A |
| ENSG00000178999 | aurora kinase B |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 24 | 81 | 54 | 98 |
| disruptive | 105 | 294 | 167 | 344 |
| missense | 64 | 228 | 138 | 266 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 36 | 164 | 73 | 122 |
| disruptive | 186 | 579 | 215 | 435 |
| missense | 102 | 438 | 170 | 295 |
PlasmoDB Total SNPs: 542
Non-coding: 260 | Synonymous: 126 | Nonsynonymous: 153 | Stop Codon: 3
Protein Length: 1650 | Molecular Weight (kDa): 193.787
UniProt ID(s): O77328
PDB ID(s): None
Isoelectric Point: 9.64
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| InterPro | IPR030616 | Aurora kinase |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 36798191 | Plasmodium ARK2-EB1 axis drives the unconventional spindle dynamics, scaffoldformation and chromosome segregation of sexual transmission stages | Zeeshan M, Rea E, ..., Tewari R | 10.21203/rs.3.rs-2539372/v1 |
| 30409996 | Inducing controlled cell cycle arrest and re-entry during asexual proliferationof Plasmodium falciparum malaria parasites | van Biljon R, Niemand J, ..., Birkholtz LM | 10.1038/s41598-018-34964-w |
| 36778504 | Plasmodium ARK2-EB1 axis drives the unconventional spindle dynamics, scaffoldformation and chromosome segregation of sexual transmission stages | Zeeshan M, Rea E, ..., Tewari R | 10.1101/2023.01.29.526106 |
| 37704606 | Plasmodium ARK2 and EB1 drive unconventional spindle dynamics, during chromosomesegregation in sexual transmission stages | Zeeshan M, Rea E, ..., Tewari R | 10.1038/s41467-023-41395-3 |