PF3D7_0309300

Genome location: Pf3D7_03_v3:397,397..401,232(+)

Genome classification: Core

Function and Localization

Product Description: N2227-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.22 (Carnosine N-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.15	0.92
Trophozoite	expressed	0.14	0.91
Schizont	expressed	0.09	0.93
Gametocyte	possibly expressed	0.34	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0309300

Resistome Missense Mutations: D623N

Resistome Compounds with Missense Mutations: BCH070

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.694 | #Insertions: 3

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0407500)

Relative Growth Rate: 0.66 ± 0.07
Confidence: 6.63

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0407500)

Modification: Disrupted | RMgm-1871

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: O77329

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.42) with 5YF0 (Global RMSD=1.47)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.22	carnosine N-methyltransferase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102486

Most Similar Human Ortholog: Q5T8V1

TM-align score: 0.92 | RMSD: 1.92

Seq Identity: 0.32 | Length: 235 / 955

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000156017	carnosine N-methyltransferase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	39	10	60
disruptive	16	78	40	118
missense	10	71	35	98

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	69	28	54
disruptive	31	168	78	188
missense	21	138	62	107

PlasmoDB Total SNPs: 257

Non-coding: 112 | Synonymous: 105 | Nonsynonymous: 40 | Stop Codon: 0

Protein Information

Protein Length: 955 | Molecular Weight (kDa): 112.248

UniProt ID(s): O77329

PDB ID(s): None

Isoelectric Point: 5.1

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003341	Cysteine rich repeat, tripleX
InterPro	IPR012901	N2227-like
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF02363	Cysteine rich repeat, tripleX
PFam	PF07942	N2227-like
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase

PMID	Title	Authors	DOI/Link
10448855	The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum.	Bowman S, Lawson D, ..., Barrell BG	10.1038/22964
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104