Product Description: asparagine synthetase [glutamine-hydrolyzing], putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.5.4 (Asparagine synthase (glutamine-hydrolyzing))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0004066 | asparagine synthase (glutamine-hydrolyzing) activity |
| Process | GO:0006529 | asparagine biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.15 | 0.49 |
| Trophozoite | expressed | 1.10 | 0.45 |
| Schizont | expressed | 0.30 | 0.81 |
| Gametocyte | possibly expressed | 0.50 | 0.72 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0309500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.521 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0407600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0407600)
Modification: Disrupted | RMgm-1872
More info: PhenoPlasm Link
AlphaFill Uniprot ID: O77330
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.5.4 | asparagine synthase (glutamine-hydrolysing) | ATPADPAMPGTPGDPCTPUTPNEMCMPUMPGMPITP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P08243 | Asparagine synthetase [glutamine-hydrolyzing] | Homo sapiens | OrthoDB, OrthoMCL BLAST | CHEMBL1627258CHEMBL1627258CHEMBL2153507CHEMBL2153507... |
Ortholog Group (OrthoMCL): OG6_100710
Most Similar Human Ortholog: P08243
TM-align score: 0.90 | RMSD: 2.05
Seq Identity: 0.39 | Length: 525 / 610
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000070669 | asparagine synthetase (glutamine-hydrolyzing) |
Protein Length: 610 | Molecular Weight (kDa): 69.864
UniProt ID(s): O77330
PDB ID(s): None
Isoelectric Point: 7.23
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001962 | Asparagine synthase |
| InterPro | IPR006426 | Asparagine synthase, glutamine-hydrolyzing |
| InterPro | IPR017932 | Glutamine amidotransferase type 2 domain |
| InterPro | IPR029055 | Nucleophile aminohydrolases, N-terminal |
| InterPro | IPR033738 | Asparagine synthase, N-terminal domain |
| PFam | PF00733 | Asparagine synthase |
| PFam | PF13537 | Glutamine amidotransferase type 2 domain |
| Superfamily | SSF52402 | Nucleophile aminohydrolases, N-terminal |
| Superfamily | SSF56235 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 34 | 29 | 48 |
| disruptive | 7 | 24 | 10 | 41 |
| missense | 5 | 23 | 9 | 34 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 82 | 39 | 37 |
| disruptive | 13 | 62 | 54 | 118 |
| missense | 9 | 49 | 41 | 72 |
PlasmoDB Total SNPs: 223
Non-coding: 206 | Synonymous: 12 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |