Product Description: calcium-dependent protein kinase 3
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.17 (Calcium/calmodulin-dependent protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0009931 | calcium-dependent protein serine/threonine kinase activity |
| Function | GO:0005516 | calmodulin binding |
| Function | GO:0004683 | calmodulin-dependent protein kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0018105 | peptidyl-serine phosphorylation |
| Process | GO:0046777 | protein autophosphorylation |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.15 | 0.92 |
| Trophozoite | possibly expressed | 0.04 | 0.98 |
| Schizont | possibly expressed | 0.02 | 0.98 |
| Gametocyte | expressed | 0.10 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0310100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -3.018 | #Insertions: 2
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1351500)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0408200)
Modification: Disrupted | RMgm-4547
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9NJU9
"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=0.09) with 2VZ6 (Global RMSD=2.06)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.17 | Ca2+/calmodulin-dependent protein kinase | NOADPGTPW13AIPEDTAEGTADTNBKN93KN62KN-93KN-62... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8IBS5 | Calcium-dependent protein kinase 4 | Plasmodium falciparum (isolate 3D7) | HOGENOM | CHEMBL2069957CHEMBL2070050CHEMBL2070050CHEMBL2070081 |
| Q5CTY5 | Calcium/calmodulin dependent protein kinase with a kinas domain and 4 calmodulin-like EF hands | OrthoDB | CHEMBL2069954CHEMBL1784959CHEMBL1784960CHEMBL1784961... | |
| Q6S4W0 | Calcium-dependent protein kinase 1 | OrthoMCL BLAST | CHEMBL2069957CHEMBL2069954CHEMBL1784959CHEMBL1784960... |
Ortholog Group (OrthoMCL): OG6_100153
Most Similar Human Ortholog: C9JEC5
TM-align score: 0.89 | RMSD: 1.17
Seq Identity: 0.25 | Length: 84 / 562
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000130822 | pregnancy up-regulated nonubiquitous CaM kinase |
| ENSG00000134072 | calcium/calmodulin dependent protein kinase I |
| ENSG00000152495 | calcium/calmodulin dependent protein kinase IV |
| ENSG00000183049 | calcium/calmodulin dependent protein kinase ID |
Protein Length: 562 | Molecular Weight (kDa): 65.288
UniProt ID(s): Q9NJU9
PDB ID(s): 3K21
Isoelectric Point: 9.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR002048 | EF-hand domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR011992 | EF-hand domain pair |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| InterPro | IPR018247 | EF-Hand 1, calcium-binding site |
| PFam | PF00069 | Protein kinase domain |
| PFam | PF13499 | EF-hand domain |
| Superfamily | SSF47473 | EF-hand domain pair |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 43 | 22 | 49 |
| disruptive | 4 | 13 | 14 | 53 |
| missense | 4 | 13 | 14 | 42 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 82 | 30 | 36 |
| disruptive | 10 | 56 | 44 | 142 |
| missense | 5 | 40 | 33 | 89 |
PlasmoDB Total SNPs: 140
Non-coding: 121 | Synonymous: 17 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10448855 | The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. | Bowman S, Lawson D, ..., Barrell BG | 10.1038/22964 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 28746405 | Biophysical characterization of the calmodulin-like domain of Plasmodiumfalciparum calcium dependent protein kinase 3. | Andresen C, Niklasson M, ..., Lundstrom P | 10.1371/journal.pone.0181721 |
| 32864371 | In-depth computational analysis of calcium-dependent protein kinase 3 ofToxoplasma gondii provides promising targets for vaccination | Majidiani H, Soltani S, ..., Foroutan M | 10.7774/cevr.2020.9.2.146 |
| 24945436 | The calcium-dependent protein kinase 3 of toxoplasma influences basal calciumlevels and functions beyond egress as revealed by quantitative phosphoproteomeanalysis | Treeck M, Sanders JL, ..., Boothroyd JC | 10.1371/journal.ppat.1004197 |
| 24514873 | Two calcium-dependent protein kinases from Chlamydomonas reinhardtii are transcriptionally regulated by nutrient starvation | Motiwalla MJ, Sequeira MP, D'Souza JS | 10.4161/psb.27969 |
| 36818849 | Calcium-dependent protein kinase CDPK16 phosphorylates serine-856 of glutamatereceptor-like GLR3.6 protein leading to salt-responsive root growth inArabidopsis() | Silamparasan D, Chang IF, Jinn TL | 10.3389/fpls.2023.1093472 |
| 24974733 | Rapid construction and screening of artificial microRNA systems in Chlamydomonasreinhardtii | Hu J, Deng X, ..., Huang K | 10.1111/tpj.12606 |
| 36265000 | A positive feedback loop mediates crosstalk between calcium, cyclic nucleotideand lipid signalling in calcium-induced Toxoplasma gondii egress | Nofal SD, Dominicus C, ..., Treeck M | 10.1371/journal.ppat.1010901 |
| 31425673 | Vaccination with recombinant Toxoplasma gondii CDPK3 induces protective immunityagainst experimental toxoplasmosis | Wu M, An R, ..., Du J | 10.1016/j.actatropica.2019.105148 |
| 30673152 | Calcium negatively regulates secretion from dense granules in Toxoplasma gondii | Katris NJ, Ke H, ..., Waller RF | 10.1111/cmi.13011 |
| 35757711 | Toxoplasma gondii CDPK3 Controls the Intracellular Proliferation of Parasites inMacrophages | Wu M, An R, ..., Du J | 10.3389/fimmu.2022.905142 |
| 16796674 | Plasmodium berghei calcium-dependent protein kinase 3 is required for ookinetegliding motility and mosquito midgut invasion | Siden-Kiamos I, Ecker A, ..., Billker O | 10.1111/j.1365-2958.2006.05189.x |
| 24176018 | Protective immunity against Toxoplasma gondii induced by DNA immunization withthe gene encoding a novel vaccine candidate: calcium-dependent protein kinase 3 | Zhang NZ, Huang SY, ..., Zhu XQ | 10.1186/1471-2334-13-512 |
| 35986017 | Live-attenuated ME49Deltacdpk3 strain of Toxoplasma gondii protects against acute andchronic toxoplasmosis | Wu M, Liu S, ..., Du J | 10.1038/s41541-022-00518-5 |
| 35142606 | Anti-Toxoplasma gondii effect of lupane-type triterpenes from the bark of blackalder (Alnus glutinosa) and identification of a potential target by reversedocking | Darme P, Escotte-Binet S, ..., Aubert D | 10.1051/parasite/2022008 |
| 35003067 | Protective Effect Against Toxoplasmosis in BALB/c Mice Vaccinated WithRecombinant Toxoplasma gondii MIF, CDPK3, and 14-3-3 Protein Cocktail Vaccine | Liu F, Wu M, ..., Du J | 10.3389/fimmu.2021.755792 |
| 38310017 | Protective effect against toxoplasmosis in BALB/C mice vaccinated withrecombinant Toxoplasma gondii CDPK3, GRA35, and ROP46 protein cocktail vaccine | Li D, Han M, ..., An R | 10.1016/j.vaccine.2024.01.050 |
| 30402958 | Characterisation of the Toxoplasma gondii tyrosine transporter and itsphosphorylation by the calcium-dependent protein kinase 3 | Wallbank BA, Dominicus CS, ..., Treeck M | 10.1111/mmi.14156 |
| 28258212 | A forward genetic screen identifies a negative regulator of rapidCa(2+)-dependent cell egress (MS1) in the intracellular parasite Toxoplasmagondii | McCoy JM, Stewart RJ, ..., Tonkin CJ | 10.1074/jbc.M117.775114 |
| 37272703 | Identification of Toxoplasma calcium-dependent protein kinase 3 as astress-activated elongation factor 2 kinase | Lis A, Baptista CG, ..., Blader IJ | 10.1128/msphere.00156-23 |